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氧化醋杆菌对D-奎尼酸及相关酸的氧化作用。

The oxidation of D-quinate and related acids by Acetomonas oxydans.

作者信息

Whiting G C, Coggins R A

出版信息

Biochem J. 1967 Jan;102(1):283-93. doi: 10.1042/bj1020283.

Abstract
  1. Growing cells of a small number of strains of Acetomonas oxydans oxidized d-quinate to 5-dehydroquinate. 2. d-Shikimate was oxidized to 4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylate (3-dehydroshikimate, formerly 5-dehydroshikimate). 3. d-Dihydroshikimate was oxidized to the corresponding 5-dehydro compound, but epidihydroshikimate oxidation by growing cells was not observed. 4. Cell-free extracts oxidized d-quinate to 5-dehydroquinate with the consumption of the stoicheiometric amount of oxygen, but oxidation of shikimate and dihydroshikimate did not go to completion. 5. Oxidation of quinate was brought about by a constitutive particulate enzyme probably localized in the cytoplasmic membrane. No evidence was found for the participation of NAD, NADP or free flavine compounds in electron transport, but the system was cytochrome-linked.
摘要
  1. 少数氧化醋酸单胞菌菌株的生长细胞可将d -奎尼酸氧化为5 -脱氢奎尼酸。2. d -莽草酸被氧化为4,5 -二羟基 - 3 -氧代环己 - 1 -烯 - 1 -羧酸(3 -脱氢莽草酸,原称5 -脱氢莽草酸)。3. d -二氢莽草酸被氧化为相应的5 -脱氢化合物,但未观察到生长细胞对表二氢莽草酸的氧化。4. 无细胞提取物将d -奎尼酸氧化为5 -脱氢奎尼酸,消耗化学计量的氧气,但莽草酸和二氢莽草酸的氧化未完全进行。5. 奎尼酸的氧化由一种组成型颗粒酶引起,该酶可能定位于细胞质膜。未发现NAD、NADP或游离黄素化合物参与电子传递的证据,但该系统与细胞色素相连。

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