Hillam R P, Tengerdy R P, Brown G L
Infect Immun. 1974 Sep;10(3):458-63. doi: 10.1128/iai.10.3.458-463.1974.
The method of Garra and Baygorria, for localized antibody production, has been adapted for obtaining high-titered and monospecific antibodies against the murine toxin of Yersinia pestis. A hollow, perforated plastic golf ball surgically implanted under the skin of rabbits induced the formation of a granuloma. When the murine toxin of Y. pestis was injected directly into the granulomatous cavity, an increased amount of antibody was found in the granuloma fluid as compared with serum or with serum antibody obtained by conventional immunization. The granuloma antibody consisted mainly of immunoglobulin G, probably produced locally by lymphoid cells of the granuloma. The immune granuloma fluid and the granuloma tissue were rich in plasma cells and lymphocytes. The chemical composition of the granuloma fluid indicated that it was a transudate.
加拉和贝戈里亚用于局部抗体产生的方法已被调整，用于获得针对鼠疫耶尔森氏菌鼠毒素的高滴度单特异性抗体。一个中空、有孔的塑料高尔夫球经手术植入兔子皮下后诱导形成肉芽肿。当将鼠疫耶尔森氏菌的鼠毒素直接注入肉芽肿腔时，与血清或通过常规免疫获得的血清抗体相比，在肉芽肿液中发现抗体量增加。肉芽肿抗体主要由免疫球蛋白G组成，可能由肉芽肿的淋巴细胞在局部产生。免疫肉芽肿液和肉芽肿组织富含浆细胞和淋巴细胞。肉芽肿液的化学成分表明它是一种漏出液。
