Tengerdy R P, Hillam R P
Bull World Health Organ. 1973;48(3):279-87.
Until now the serological typing of Yersinia pestis into subgroups has not proved possible because all the antigenic components are present in each isolate. Using acrylamide disk electrophoresis it was observed that differences exist in the protein distribution patterns of aqueous extracts from various Y. pestis isolates. One component that was especially plentiful in some Javanese and South American isolates was identified as the murine toxin. The amount of murine toxin varies significantly from isolate to isolate, and so permits them to be roughly grouped. In 28 degrees C cultures the variation in the murine toxin content is independent of the variation in the F-I content. A new method of typing based on this 2-trait variation in quantities, in contrast to classical typing based on qualitative differences, might prove to be a means of differentiating Y. pestis isolates.
到目前为止，鼠疫耶尔森氏菌血清学分型为亚群尚未被证明是可行的，因为所有抗原成分都存在于每一个分离株中。通过丙烯酰胺圆盘电泳观察到，不同鼠疫耶尔森氏菌分离株水提取物的蛋白质分布模式存在差异。在一些爪哇和南美分离株中特别丰富的一种成分被鉴定为鼠毒素。鼠毒素的含量在不同分离株之间差异显著，因此可以将它们大致分组。在28摄氏度培养条件下，鼠毒素含量的变化与F-I含量的变化无关。与基于定性差异的经典分型相比，基于这两种数量性状变化的新分型方法可能被证明是区分鼠疫耶尔森氏菌分离株的一种手段。
