Deven J M, Manocha M S
Can J Microbiol. 1976 Apr;22(4):443-9. doi: 10.1139/m76-070.
The fatty acid composition of the total and polar lipid fractions of Choanephora cucurbitarum grown under different cultural conditions were analyzed by thin-layer and gas-liquid chromatography. It was observed that temperature, age, pH, and light influenced the degree of unsaturation, this being due mainly to changes in the gamma-linolenic acid concentration. The conditions used in this study did not alter the qualitative profile of fatty acids normally present in the organism. Neither did these conditions stimulate the production of further long-chain fatty acids (C20-C26) beyond gamma-linolenic acid (C18:3) as reported earlier using growth media containing glutamic acid. The fatty acid pattern of lipid fractions though the same qualitatively, differed quantitatively. The polar lipid fractions, phosphatidyl choline, phosphatidyl ethanolamine, and diphosphatidyl glycerol showed an appreciable variation in gamma-linolenic acid content under different cultural conditions. The degree of unsaturation of the various lipid fractions decreased with increases in temperature, light intensity, and pH, but within each treatment the same pattern of decreasing degree of unsaturation with increasing age was observed. The significance of these observations is discussed.
采用薄层色谱法和气液色谱法，分析了在不同培养条件下生长的瓜笄霉的总脂质部分和极性脂质部分的脂肪酸组成。结果发现，温度、菌龄、pH值和光照会影响不饱和度，这主要是由于γ-亚麻酸浓度的变化所致。本研究采用的条件并未改变该生物体中正常存在的脂肪酸定性分布。这些条件也没有像之前使用含谷氨酸的生长培养基报道的那样，刺激产生超过γ-亚麻酸（C18:3）的更多长链脂肪酸（C20 - C26）。脂质部分的脂肪酸模式虽然在定性上相同，但在定量上有所不同。极性脂质部分，即磷脂酰胆碱、磷脂酰乙醇胺和双磷脂酰甘油，在不同培养条件下γ-亚麻酸含量呈现出明显变化。各种脂质部分的不饱和度随着温度、光照强度和pH值的升高而降低，但在每种处理中，均观察到随着菌龄增加不饱和度降低的相同模式。讨论了这些观察结果的意义。
