Seawright G L, Harding G, Thomas F C, Hanson R P
Appl Microbiol. 1974 Nov;28(5):802-6. doi: 10.1128/am.28.5.802-806.1974.
A microculture plaque neutralization test is described for California-group arboviruses that is as precise and quantitative as the standard test conducted in 60-mm petri dishes. It was shown that there was no significant between-panel or between-day variation in determinations and that a single pipette could be used for all serum-dilution levels within a titration without inoculum carry-over effect. The experimental protocol and statistical methods used produce 50% neutralization end points that meet the assumptions of parametric statistics. This permits the power and versatility of the analysis of variance to be exploited in testing for treatment effects in serological and immunological studies with viruses.
本文描述了一种针对加利福尼亚组虫媒病毒的微量培养蚀斑中和试验，该试验与在60毫米培养皿中进行的标准试验一样精确且可定量。结果表明，不同试验组之间或不同日期的测定结果无显著差异，并且在滴定过程中，同一移液器可用于所有血清稀释水平，不存在接种物残留效应。所采用的实验方案和统计方法产生的50%中和终点符合参数统计的假设。这使得方差分析的功效和通用性可用于病毒血清学和免疫学研究中的治疗效果测试。
