线粒体核糖体与胞质核糖体。区分特征以及蛋白质合成对同源核糖体盐可提取部分的需求。
Mitochondrial and cytoplasmic ribosomes. Distinguishing characteristics and a requirement for the homologous ribosomal salt-extractable fraction for protein synthesis.
作者信息
Avadhani N G, Buetow D E
出版信息
Biochem J. 1974 Apr;140(1):73-8. doi: 10.1042/bj1400073.
Abstract
- Mitochondrial and cytoplasmic ribosomes of Euglena gracilis differ in their total RNA and protein content. 2. Mitochondrial ribosomes dissociate to subunits at higher Mg(2+) concentrations than do cytoplasmic ribosomes. 3. A separable 5S RNA is obtained from cytoplasmic and chloroplast ribosomes, but not from mitochondrial ribosomes. 4. For protein-synthesizing activity with a natural mRNA, mitochondrial ribosomes use tRNA from any cell compartment and are partly active with supernatant enzymes from cytoplasm. Cytoplasmic ribosomes are partly active with enzymes and tRNA from mitochondria or chloroplasts. 5. Both mitochondrial and cytoplasmic ribosomes show high specificity for the homologous salt-extractable ribosomal fraction for protein-synthesizing activity.
摘要
- 纤细裸藻的线粒体核糖体和细胞质核糖体在总RNA和蛋白质含量上存在差异。2. 线粒体核糖体在比细胞质核糖体更高的Mg(2+)浓度下解离为亚基。3. 可从细胞质核糖体和叶绿体核糖体中获得可分离的5S RNA，但不能从线粒体核糖体中获得。4. 对于使用天然mRNA的蛋白质合成活性，线粒体核糖体使用来自任何细胞区室的tRNA，并且与来自细胞质的上清酶部分具有活性。细胞质核糖体与来自线粒体或叶绿体的酶和tRNA部分具有活性。5. 线粒体核糖体和细胞质核糖体在蛋白质合成活性方面对同源盐可提取核糖体部分均表现出高度特异性。
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Mitochondrial and cytoplasmic ribosomes. Distinguishing characteristics and a requirement for the homologous ribosomal salt-extractable fraction for protein synthesis.线粒体核糖体与胞质核糖体。区分特征以及蛋白质合成对同源核糖体盐可提取部分的需求。
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