赖氨酰和苯丙氨酰转移核糖核酸合成酶的多核糖体及颗粒分布
Polyribosomal and particulate distribution of lysyl- and phenylalanyl-transfer ribonucleic acid synthetases.
作者信息
Moline G, Hampel A, Enger M D
出版信息
Biochem J. 1974 Oct;143(1):191-5. doi: 10.1042/bj1430191.
Abstract
- Only two aminoacyl-tRNA synthetases from Chinese hamster ovary cells are found associated with ribosomes and polyribosomes. 2. Phenylalanyl-tRNA synthetase activity is found with the 60S subunit, 80S monoribosome and individual polyribosomes. An additional 15S form of the enzyme is also seen. 3. Lysyl-tRNA synthetase activity is found in a form of about 20S and associated with ribosomal subunits and polyribosomes. The ribosomal subunits having lysyl-tRNA synthetase activity are about 6S larger than the bulk of the ribosomal subunits. 4. The lysyl- and phenylalanyl-tRNA synthetases found in different complexes have differential sensitivity to EDTA and centrifugation properties.
摘要
- 在中国仓鼠卵巢细胞中，仅发现两种氨酰-tRNA合成酶与核糖体和多核糖体相关联。2. 苯丙氨酰-tRNA合成酶活性存在于60S亚基、80S单核糖体和单个多核糖体中。还可见到该酶的另一种15S形式。3. 赖氨酰-tRNA合成酶活性以约20S的形式存在，并与核糖体亚基和多核糖体相关联。具有赖氨酰-tRNA合成酶活性的核糖体亚基比大多数核糖体亚基大约6S。4. 在不同复合物中发现的赖氨酰-和苯丙氨酰-tRNA合成酶对EDTA具有不同的敏感性和离心特性。
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