Rijke R P, Gart R
Virchows Arch B Cell Pathol Incl Mol Pathol. 1979;31(1):15-22. doi: 10.1007/BF02889918.
Epithelial cell loss was induced in the descending colon of the rat by temporary ischaemia to investigate whether this would lead to an increase in crypt cell proliferation. Shortly after the temporary ischaemia the number of cells per crypt was markedly reduced, and it was shown that the cell loss occurred mainly from the non-proliferating upper half of the crypt. The number of cells per crypt reached control values again after 24-48 h. There was a marked increase in proliferative activity, as reflected by the labelling index after 3HTdR and by the mitotic index, with peak values at 16 and 24 h after ischaemia. After 48 h the proliferative indices were normal again. The increase in crypt cell proliferation was characterized by an increase in the labelling index as well as in the mitotic index per crypt cell position. No enlargement of the proliferative cell compartment in the crypt was observed. It is most likely then that the increase in crypt cell proliferation was brought about by a shortening of the cell cycle, since the growth fraction in the lower half of the crypt approaches 1.0. The possible implications of the present data for the control of colonic cell proliferation and colonic carcinogenesis are discussed.
通过短暂缺血诱导大鼠降结肠上皮细胞丢失，以研究这是否会导致隐窝细胞增殖增加。短暂缺血后不久，每个隐窝的细胞数量明显减少，并且表明细胞丢失主要发生在隐窝的非增殖性上半部分。每个隐窝的细胞数量在24 - 48小时后再次达到对照值。增殖活性显著增加，这通过3HTdR后的标记指数和有丝分裂指数得以体现，在缺血后16小时和24小时达到峰值。48小时后增殖指数再次恢复正常。隐窝细胞增殖的增加表现为每个隐窝细胞位置的标记指数以及有丝分裂指数的增加。未观察到隐窝中增殖细胞区室的扩大。那么很可能隐窝细胞增殖的增加是由细胞周期缩短引起的，因为隐窝下半部分的生长分数接近1.0。讨论了本研究数据对结肠细胞增殖控制和结肠癌发生的可能影响。
