Rijke R P, Gart R, Langendoen N J
Virchows Arch B Cell Pathol Incl Mol Pathol. 1979;31(1):23-30. doi: 10.1007/BF02889919.
The influence of experimental bypass on the epithelial cell kinetics in the rat descending colon was studied. It was found that the number of cells per crypt was markedly reduced at 6 weeks after bypass. The percentage of labelled crypt cells, 1 h after 3HTdR, and the distribution of labelled cells in the crypt was normal. Also the life span of the epithelial cells was the same in control and bypassed colon. The response of crypt cell proliferation to ischaemia-induced cell loss in the bypassed descending colon was similar to the one previously described for normal descending colon. This indicates that the absence of the normal luminal contents does not result in a different response of colonic crypts to induced cell loss. Furthermore, it was found that the number of cells per crypt and the proliferative activity did not change in the transverse colon after temporary ischaemia of the bypassed descending colon. This indicates that the increase in crypt cell proliferation after ischaemia-induced cell loss is a local response.
研究了实验性旁路对大鼠降结肠上皮细胞动力学的影响。发现旁路术后6周时，每个隐窝的细胞数量显著减少。给予3HTdR 1小时后，标记隐窝细胞的百分比以及标记细胞在隐窝中的分布是正常的。此外，对照结肠和旁路结肠中上皮细胞的寿命相同。旁路降结肠中隐窝细胞增殖对缺血诱导的细胞丢失的反应与先前描述的正常降结肠相似。这表明正常腔内内容物的缺失不会导致结肠隐窝对诱导性细胞丢失产生不同反应。此外，发现旁路降结肠暂时缺血后，横结肠中每个隐窝的细胞数量和增殖活性没有变化。这表明缺血诱导的细胞丢失后隐窝细胞增殖的增加是一种局部反应。
