Wong E, Schousboe A, Saito K, Wu J Y, Roberts E
Brain Res. 1974 Mar 15;68(1):133-42. doi: 10.1016/0006-8993(74)90538-1.
Six different inbred strains of mice (C57BL/6J, CBA/CaJ, CE/J, DBA/2J, LP/J and RF/J) were compared in terms of specific activities and immunochemical properties of brain L-glutamate decarboxylase (GAD) and gamma-aminobutyrate transaminase (GABA-T), the enzymes responsible for the synthesis and degradation of GABA, respectively. GAD from the brains of the different strains was indistinguishable on the basis of specific activities, double diffusion tests, immunoelectrophoresis and inhibition by antibody. However, microcomplement fixation tests showed GAD from DBA and C57BL mice to be most distinctly different from GAD extracted from the Swiss mouse, from which the original antigen was prepared and that the enzyme from the CE, LP and RF also differed. Similar fixation curves were obtained for the GAD from CBA and Swiss mice. GABA-T from the different strains was indistinguishable on the basis of all the tests employed.
对六种不同的近交系小鼠（C57BL/6J、CBA/CaJ、CE/J、DBA/2J、LP/J和RF/J）的脑L-谷氨酸脱羧酶（GAD）和γ-氨基丁酸转氨酶（GABA-T）的比活性和免疫化学特性进行了比较，这两种酶分别负责GABA的合成和降解。不同品系小鼠脑内的GAD，在比活性、双向扩散试验、免疫电泳及抗体抑制方面无明显差异。然而，微量补体结合试验表明，DBA和C57BL小鼠的GAD与从制备原始抗原的瑞士小鼠中提取的GAD差异最为明显，CE、LP和RF小鼠的酶也存在差异。CBA和瑞士小鼠的GAD获得了相似的结合曲线。在所采用的所有试验中，不同品系小鼠的GABA-T无明显差异。
