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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1965 Aug;96(2):404-12. doi: 10.1042/bj0960404.

The distribution of adenosine triphosphatase was studied in morphologically characterized subcellular fractions of guinea-pig brain. The conditions of homogenization were selected so as to favour the survival of nerve endings as organized structures. 2. A fraction consisting mainly of the external membranes of nerve endings was rich in a ouabain-sensitive Na(+)-K(+)-stimulated adenosine triphosphatase which closely resembled that present in the classical microsomal fraction studied by other workers, but which showed a higher specific activity. 3. A dinitrophenol-stimulated adenosine triphosphatase was located in the nerve-ending mitochondria. 4. The synaptic-vesicle fraction contained a small amount of adenosine triphosphatase that differed in its response to several ions and other compounds from the membrane, myelin and mitochondrial fractions, indicating freedom from contamination by these elements.

研究了豚鼠脑形态学特征明确的亚细胞组分中三磷酸腺苷酶的分布。选择匀浆条件以利于神经末梢作为有组织结构的存活。2. 一个主要由神经末梢外膜组成的组分富含哇巴因敏感的钠钾刺激型三磷酸腺苷酶，它与其他研究者研究的经典微粒体组分中的酶非常相似，但具有更高的比活性。3. 二硝基苯酚刺激型三磷酸腺苷酶位于神经末梢线粒体中。4. 突触小泡组分含有少量三磷酸腺苷酶，其对几种离子和其他化合物的反应与膜、髓鞘和线粒体组分不同，表明未受这些成分的污染。

Biochem J. 1965 Aug;96(2):404-12. doi: 10.1042/bj0960404.

The distribution of adenosine triphosphatase was studied in morphologically characterized subcellular fractions of guinea-pig brain. The conditions of homogenization were selected so as to favour the survival of nerve endings as organized structures. 2. A fraction consisting mainly of the external membranes of nerve endings was rich in a ouabain-sensitive Na(+)-K(+)-stimulated adenosine triphosphatase which closely resembled that present in the classical microsomal fraction studied by other workers, but which showed a higher specific activity. 3. A dinitrophenol-stimulated adenosine triphosphatase was located in the nerve-ending mitochondria. 4. The synaptic-vesicle fraction contained a small amount of adenosine triphosphatase that differed in its response to several ions and other compounds from the membrane, myelin and mitochondrial fractions, indicating freedom from contamination by these elements.

研究了豚鼠脑形态学特征明确的亚细胞组分中三磷酸腺苷酶的分布。选择匀浆条件以利于神经末梢作为有组织结构的存活。2. 一个主要由神经末梢外膜组成的组分富含哇巴因敏感的钠钾刺激型三磷酸腺苷酶，它与其他研究者研究的经典微粒体组分中的酶非常相似，但具有更高的比活性。3. 二硝基苯酚刺激型三磷酸腺苷酶位于神经末梢线粒体中。4. 突触小泡组分含有少量三磷酸腺苷酶，其对几种离子和其他化合物的反应与膜、髓鞘和线粒体组分不同，表明未受这些成分的污染。