Salvaterra P M, Matthews D A
Neurochem Res. 1980 Feb;5(2):181-95. doi: 10.1007/BF00964331.
A simple reliable method was developed for the rapid isolation of a synaptic plasma membrane-enriched fraction from rat brain. The procedure involves the direct lysis of a crude mitochondrial fraction followed by a combined flotation-sedimentation density gradient centrifugation in a fixed-angle centrifuge rotor. All fractions have been characterized with respect to relative enrichment of (Na+-K+)ATPase activity as well as putative cholinergic neurotransmitter receptors determined by [125I]alpha-bungarotoxin and [3H]quinuclidinyl benzilate binding. The 2- to 4-fold relative enrichment of putative receptor binding sites correlated well with the 4-fold enrichment of morphologically identifiable synaptic junctions in the synaptic plasma membrane enriched fraction.
已开发出一种简单可靠的方法，用于从大鼠脑中快速分离富含突触质膜的组分。该程序包括直接裂解粗线粒体组分，然后在固定角度离心机转子中进行浮选 - 沉降密度梯度离心。所有组分均已根据（Na + -K +）ATP酶活性的相对富集以及通过[125I]α-银环蛇毒素和[3H]喹核醇基苯甲酸酯结合测定的假定胆碱能神经递质受体进行了表征。假定受体结合位点2至4倍的相对富集与富含突触质膜的组分中形态学上可识别的突触连接4倍的富集密切相关。
