脑微粒体的二磷酸腺苷-三磷酸腺苷交换反应及其与钠离子刺激的三磷酸腺苷酶反应的关系。
The adenosine diphosphate--adenosine triposphate-excange reaction of cerebral microsomes and its relation to he sodium ion-stimulatd adenosine-triphosphatase reaction.
作者信息
Swanson P D, Stah W L
出版信息
Biochem J. 1966 May;99(2):396-403. doi: 10.1042/bj0990396.
Abstract
Microsomes from guinea-pig cerebral cortex contain a system capable of exchanging ADP with ATP at rates of about 20mumoles/mg. of protein/hr. The ADP-ATP-exchange reaction requires Mg(2+) for activity. The reaction is not stimulated by Na(+) or K(+) and is not inhibited by ouabain, in contrast with the Na(+)-plus-K(+)-stimulated adenosine triphosphatase. The pH optimum also differs from that of the adenosine triphosphatase. The ADP-ATP-exchange reaction is stimulated two- to three-fold by non-ionic, anionic and cationic detergents, even when these agents are inhibiting the adenosine-triphosphatase reaction. This reaction may represent a component of the Na(+)-plus-K(+)-stimulated adenosine-triphosphatase reaction but is more likely to be due to other enzyme systems present in microsomal subfractions.
摘要
豚鼠大脑皮层的微粒体含有一个系统，该系统能够以约20微摩尔/毫克蛋白质/小时的速率进行ADP与ATP的交换。ADP-ATP交换反应需要Mg(2+)来发挥活性。与Na(+)加K(+)刺激的腺苷三磷酸酶不同，该反应不受Na(+)或K(+)的刺激，也不受哇巴因的抑制。最适pH值也与腺苷三磷酸酶不同。即使这些试剂抑制腺苷三磷酸酶反应，非离子、阴离子和阳离子去污剂也能将ADP-ATP交换反应刺激两到三倍。该反应可能代表Na(+)加K(+)刺激的腺苷三磷酸酶反应的一个组成部分，但更可能是由于微粒体亚组分中存在的其他酶系统所致。
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