气相色谱法在胃粘多糖水解产物中中性单糖分析中的应用。
The use of gas-liquid chromatography in the analysis of neutral monosaccharides in hydrolysates of gastric mucopolysaccharides.
作者信息
Oates M D, Schrager J
出版信息
Biochem J. 1965 Dec;97(3):697-700. doi: 10.1042/bj0970697.
Abstract
- Conditions are described for the separation and estimation of the neutral monosaccharides obtained on acidic hydrolysis of human gastric mucopolysaccharides. 2. The technique involves the formation of the trimethylsilyl derivatives of the sugars and the analysis of these by gas-liquid chromatography. 3. The monosaccharides estimated in gastric mucopolysaccharides by this technique were l-fucose, d-mannose, d-galactose and d-glucose. 4. The analytical values for glucose and fucose obtained by this method agreed well with values obtained by the glucose oxidase and thioglycollic acid methods respectively. 5. Evidence is presented which clearly indicates that gas-liquid chromatography is a faster, more sensitive and more convenient technique for the measurement of these compounds than any other in use at present.
摘要
- 描述了分离和估算人胃粘多糖酸性水解后得到的中性单糖的条件。2. 该技术包括糖类三甲基硅烷基衍生物的形成以及通过气液色谱法对其进行分析。3. 通过该技术估算的胃粘多糖中的单糖为L-岩藻糖、D-甘露糖、D-半乳糖和D-葡萄糖。4. 用该方法获得的葡萄糖和岩藻糖的分析值分别与用葡萄糖氧化酶法和巯基乙酸法获得的值吻合良好。5. 所提供的证据清楚地表明，对于这些化合物的测量，气液色谱法是一种比目前使用的任何其他方法都更快、更灵敏且更方便的技术。
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The use of gas-liquid chromatography in the analysis of neutral monosaccharides in hydrolysates of gastric mucopolysaccharides.气相色谱法在胃粘多糖水解产物中中性单糖分析中的应用。
Biochem J. 1965 Dec;97(3):697-700. doi: 10.1042/bj0970697.
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本文引用的文献
1
QUANTITATIVE ANALYSIS OF SERUM AND URINE SUGARS BY GAS CHROMATOGRAPHY.气相色谱法对血清和尿液糖分的定量分析
Clin Chim Acta. 1964 Oct;10:352-9. doi: 10.1016/0009-8981(64)90066-x.
2
THE SEPARATION, DETERMINATION, AND CHARACTERIZATION OF 2-AMINO-2-DEOXY-D-GLUCOSE (D-GLUCOSAMINE) AND 2-AMINO-2-DEOXY-D-GALACTOSE (D-GALACTOSAMINE) IN BIOLOGICAL MATERIALS BY GAS-LIQUID PARTITION CHROMATOGRAPHY.用气相分配色谱法分离、测定和鉴定生物材料中的2-氨基-2-脱氧-D-葡萄糖（D-葡糖胺）和2-氨基-2-脱氧-D-半乳糖（D-半乳糖胺）
Can J Biochem. 1964 Apr;42:451-60. doi: 10.1139/o64-053.
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THE USE OF GAS-LIQUID CHROMATOGRAPHY IN THE ASSAY OF SOME CORTICOSTEROIDS IN URINE.气相色谱法在尿液中某些皮质类固醇测定中的应用
J Endocrinol. 1964 Jan;28:131-8. doi: 10.1677/joe.0.0280131.
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An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels.一种用于测定血液、脑脊液和尿液葡萄糖水平的改良葡萄糖氧化酶法。
Clin Chim Acta. 1959 May;4(3):395-400. doi: 10.1016/0009-8981(59)90110-x.
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