Huggins K G, Roitt I M, Brostoff J, Taylor W A
Int Arch Allergy Appl Immunol. 1979;58(3):241-50. doi: 10.1159/000232200.
An 131I-labelled IgE myeloma, standardized against the WHO reference serum for IgE was bound in varying concentrations to discs bearing anti-human light chain. These were used to construct a calibration curve for the subsequent binding of 125I-labelled anti-IgE, thus relating 125I counts to WHO IgE units. The calibrated anti-IgE could then be used to measure the actual amount of specific IgE bound to an allergen disc in the radioallergosorbent test. Using this standardized system, soluble allergen extracts could then be assayed by their ability to inhibit the binding of a defined amount of specific IgE, thereby enabling their activity to be expressed in WHO IgE units.
一种以世界卫生组织IgE参考血清标准化的131I标记的IgE骨髓瘤，以不同浓度与带有抗人轻链的圆盘结合。这些圆盘用于构建校准曲线，以用于后续125I标记的抗IgE的结合，从而将125I计数与世界卫生组织IgE单位相关联。然后，校准后的抗IgE可用于在放射变应原吸附试验中测量与变应原圆盘结合的特异性IgE的实际量。使用这个标准化系统，可溶性变应原提取物可以通过其抑制一定量特异性IgE结合的能力进行测定，从而使其活性能够以世界卫生组织IgE单位表示。
