Schmidt R R, Kaplan S, Smith J J
J Appl Physiol Respir Environ Exerc Physiol. 1979 Feb;46(2):298-301. doi: 10.1152/jappl.1979.46.2.298.
Stage 34 (8-day) and 41 (15-day) chick embryos (Gallus gallus; in ovo) were exposed (3 min) to two separate amounts of mechanical (shaking) stress. Oxygen consumption determinations, following a temperature equilibration period (60 min), were made on entire, intact eggs using a Warburg apparatus equipped with 130-ml flasks modified to hold the egg. Shaking at 300 excursions per minute (epm) resulted in depressed O2 consumption by stages 34 and 41. Only stage 34 embryos had markedly depressed O2 consumption values when shaken at 100 epm. When exposed to 300 epm and reincubated for either 1, 2, or 4 hr prior to O2 consumption determinations stage 34 embryos, following an early return to control levels exhibited a marked fall in O2 consumption by 4 h reincubation. The stage 41 embryos, on the other hand, demonstrated a gradual rise to control O2 consumption levels by 4 h reincubation. Oxygen consumption has now been shown for the first time in an embryonic system (at two distinct developmental stages) to be functionally impaired by mechanically induced stress.
将第34阶段（8日龄）和第41阶段（15日龄）的鸡胚胎（原鸡；在卵内）暴露于两种不同强度的机械（摇晃）应激下3分钟。在温度平衡期（60分钟）后，使用配备有经改装以容纳鸡蛋的130毫升烧瓶的瓦氏呼吸仪对完整的鸡蛋进行耗氧量测定。每分钟300次摆动（epm）的摇晃导致第34和41阶段的氧气消耗量降低。只有第34阶段的胚胎在以100 epm摇晃时氧气消耗量明显降低。当第34阶段的胚胎暴露于300 epm并在耗氧量测定前再孵化1、2或4小时时，在早期恢复到对照水平后，再孵化4小时时氧气消耗量明显下降。另一方面，第41阶段的胚胎在再孵化4小时时显示出逐渐上升至对照耗氧量水平。耗氧量现已首次在胚胎系统中（在两个不同的发育阶段）被证明因机械诱导的应激而受到功能损害。
