Raddatz E, Eyal-Giladi H, Kucera P
J Exp Zool Suppl. 1987;1:213-8.
Oxygen uptake was studied during the establishment of cephalocaudal polarity in the very early chick embryo, i.e., 10 hr before (stage VI) and at laying (stage X). Oxygen fluxes in minute regions of the intact blastoderms were measured in vitro by scanning microspectrophotometry in the presence or absence of glucose. The oxygen consumption of the whole blastoderm remained constant (6 nmol O2 X hr-1) throughout the period studied, although the number of cells increased more than twofold. The regional oxygen fluxes varied from 0.41 to 1.13 nmol O2 X hr-1 X mm-2 at stage VI and from 0.42 to 0.70 nmol O2 X hr-1 X mm-2 at stage X. At stage VI, the oxygen flux in the center of the blastoderm was significantly higher than that in its periphery. This pattern remained evident when the values were corrected for cell number or for cytoplasmic volume. At stage X, there was a tendency for the oxygen fluxes to decrease from the posterior to the anterior regions of the area pellucida. Thus the pattern of oxidative metabolism in the late uterine embryos seems to change from radial to bilateral. This change of symmetry probably reflects the process of formation of the embryonic axis. In addition, the fact that the oxygen uptake was similar in the presence or absence of glucose suggests that early chick embryos metabolize essentially intracellular stores.
在鸡胚发育极早期，即产卵前10小时（VI期）和产卵时（X期），研究了头尾极性建立过程中的氧摄取情况。通过扫描显微分光光度法，在有或无葡萄糖存在的情况下，体外测量完整胚盘微小区域的氧通量。在所研究的整个时期内，尽管细胞数量增加了两倍多，但整个胚盘的氧消耗保持恒定（6 nmol O₂·hr⁻¹）。VI期区域氧通量在0.41至1.13 nmol O₂·hr⁻¹·mm⁻²之间，X期在0.42至0.70 nmol O₂·hr⁻¹·mm⁻²之间。在VI期，胚盘中心的氧通量显著高于其周边。当对细胞数量或细胞质体积进行校正后，这种模式仍然明显。在X期，透明区从后部到前部区域的氧通量有降低的趋势。因此，晚期子宫内胚胎的氧化代谢模式似乎从径向变为双侧。这种对称性的变化可能反映了胚胎轴的形成过程。此外，无论有无葡萄糖存在，氧摄取都相似，这一事实表明早期鸡胚主要利用细胞内储存物质进行代谢。
