Blanck J, Rohde K, Ruckpaul K
Acta Biol Med Ger. 1979;38(1):23-32.
The aerobic NADPH reduction of the Triton N-101 disintegrated cytochrome P-450LM system has been studied. At this organization level--the components being dispersed in solution--a first-order monophasic reaction is exhibited. Neither the complex formation of cytochrome and reductase, respectively, nor preceding diffusion is rate limiting. The initial rate follows the ratio reductase/P-450 (mole/mole) thus indicating a Michaelis-Menten type enzyme mechanism. A model treatment of the reaction fits the systems behaviour as a whole. A multiparameter equilibrium state and different specified time function equations were developed for the determination of individual step rate constants and other system parameters as well.
对Triton N - 101裂解的细胞色素P - 450LM系统的需氧NADPH还原反应进行了研究。在这个组织水平上——各组分分散在溶液中——呈现出一级单相反应。细胞色素和还原酶之间的复合物形成以及之前的扩散均不是限速步骤。初始速率遵循还原酶/P - 450（摩尔/摩尔）的比例，因此表明是米氏酶机制。对该反应的模型处理符合整个系统的行为。还开发了多参数平衡态和不同的特定时间函数方程，用于确定各个步骤的速率常数以及其他系统参数。
