膜脂的物理状态调节补体第一成分的激活。

The physical state of membrane lipids modulates the activation of the first component of complement.

作者信息

Esser A F, Bartholomew R M, Parce J W, McConnell H M

出版信息

J Biol Chem. 1979 Mar 25;254(6):1768-70.

Abstract

Activation of the first component of human complement (C1) by bilayer-embedded nitroxide spin label lipid haptens and specific rabbit antinitroxide antibody has been measured. The nitroxide spin label hapten was contained in host bilayers of either dimyristoyl phosphatidylcholine or dipalmitoyl phosphatidylcholine in the form of both liposomes and vesicles. At a temperature of 32 degrees C, which is intermediate between the hydrocarbon chain-melting temperatures of the two phospholipids, activation of C1 in such vesicles and liposomes is more efficient in the fluid membrane. Studies of C1 activation in binary mixtures of cholesterol and dipalmitoyl phosphatidylcholine indicate that the activation of C1 is not limited by the lateral diffusion of the lipid haptens in these membranes.

摘要

已对双层嵌入的氮氧化物自旋标记脂质半抗原和特异性兔抗氮氧化物抗体激活人补体第一成分（C1）的情况进行了测定。氮氧化物自旋标记半抗原以脂质体和囊泡的形式存在于二肉豆蔻酰磷脂酰胆碱或二棕榈酰磷脂酰胆碱的主体双层中。在32℃的温度下（该温度介于两种磷脂的烃链熔化温度之间），在这种囊泡和脂质体中，C1在流体膜中的激活效率更高。对胆固醇和二棕榈酰磷脂酰胆碱二元混合物中C1激活的研究表明，C1的激活不受脂质半抗原在这些膜中横向扩散的限制。