Painter G M, Deyoe B L, Lambert G
Can J Comp Med Vet Sci. 1966 Aug;30(8):218-23.
Experiments were conducted to compare the efficacy of several media to isolate Br. abortus, Br. suis, and Br. melitensis from infected animal tissues. Greater differences among media were observed when infected tissues were the source of organisms than when in vitro propagated strains were used. All basal, enriched, and selective media used were adequate for isolation in most instances. However, enrichment of media with serum was necessary for maximum isolation of all strains of Brucella. Due to Brucella species and strain differences, employment of more than one medium was advantageous.
进行了多项实验,以比较几种培养基从受感染动物组织中分离流产布鲁氏菌、猪布鲁氏菌和羊布鲁氏菌的效果。与使用体外繁殖菌株相比,当受感染组织作为微生物来源时,各培养基之间的差异更为明显。在大多数情况下,所有使用的基础培养基、富集培养基和选择性培养基都足以用于分离。然而,为了最大程度地分离所有布鲁氏菌菌株,培养基中添加血清进行富集是必要的。由于布鲁氏菌的种类和菌株存在差异,使用一种以上的培养基是有利的。