Fiorentino M A, Cipolla A L, Malena C R, Paolicchi F A
Laboratorio de Bacteriología, Grupo de Sanidad Animal, Unidad Integrada Balcarce, EEA INTA-Facultad de Ciencias Agrarias UNMdP, CC276, 7620 Balcarce, Argentina.
Rev Argent Microbiol. 2003 Jul-Sep;35(3):123-7.
The diagnostic efficiency of two hemoculture media for the detection of different species of Brucella strains was evaluated. Strains of Brucella melitensis, Brucella suis, Brucella abortus, Brucella ovis, and Brucella abortus S19 were used. Each strain was diluted in phosphate buffer saline (PBS) to obtain a concentration of 10(5) colony forming units/ml (CFU/ml). Blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 10(3) CFU/ml. These blood samples were inoculated into the following media: (i) Hemobrucella (HB), (ii) Tryptose citrated broth 2% (CTB), and (iii) Controls without blood for B. melitensis and B.suis. Subculture in dishes and CFU/ml counts were made at the 1st, 3rd, 8th, 10th, 20th, and 30th post-inoculation (PI) day. Best results were obtained in the HB medium for all strains, except for B. suis, which due to the presence of a contaminant did not reach its maximum development in this medium. All strains were recovered from both media at 24 h PI, except B. ovis that was isolated from HB at 72 h PI and was not recovered from CTB. All strains remained viable for a shorter period in CTB. Under the proposed experimental conditions the HB medium was more sensitive than CTB. Future experiments should evaluate the utility of this commercial medium in clinical cases of animal brucellosis.
评估了两种血培养培养基对不同种布鲁氏菌菌株的诊断效率。使用了羊种布鲁氏菌、猪种布鲁氏菌、牛种布鲁氏菌、绵羊种布鲁氏菌和牛种布鲁氏菌S19菌株。将每种菌株在磷酸盐缓冲盐水（PBS）中稀释，以获得浓度为10⁵菌落形成单位/毫升（CFU/毫升）。将山羊、猪、牛和绵羊的血液与细菌悬液混合，以获得最终浓度小于或等于10³CFU/毫升。将这些血液样本接种到以下培养基中：（i）血布鲁氏菌培养基（HB），（ii）2%胰蛋白胨柠檬酸盐肉汤（CTB），以及（iii）针对羊种布鲁氏菌和猪种布鲁氏菌的无血对照。在接种后第1、3、8、10、20和30天进行平板传代培养并计数CFU/毫升。除猪种布鲁氏菌外，所有菌株在HB培养基中均获得最佳结果，由于存在污染物，猪种布鲁氏菌在该培养基中未达到最大生长。在接种后24小时，所有菌株均可从两种培养基中分离得到，但绵羊种布鲁氏菌在接种后72小时才从HB培养基中分离得到，且未从CTB培养基中分离得到。所有菌株在CTB中存活的时间较短。在所提出的实验条件下，HB培养基比CTB更敏感。未来的实验应评估这种商业培养基在动物布鲁氏菌病临床病例中的效用。
