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钠钾腺苷三磷酸酶：酰基磷酸“中间体”被证明是L-谷氨酰-γ-磷酸。

Sodium-potassium adenosine triphosphatase: acyl phosphate "intermediate" shown to be L-glutamyl-gamma-phosphate.

作者信息

Kahlenberg A, Galsworthy P R, Hokin L E

出版信息

Science. 1967 Jul 28;157(3787):434-6. doi: 10.1126/science.157.3787.434.

DOI:10.1126/science.157.3787.434
PMID:4226245
Abstract

Peptides obtained from pepsin digestion of the phosphorylated and nonphosphorylated forms of a preparation of brain microsomal sodium-potassium-activated adenosine triphosphatase were treated at pH 5.4 with N-(n-propyl-2,3-(3)H) hydroxylamine of high specific activity, then separated by column chromatography, and further digested with pronase. A compound isolated in higher amounts from the phosphorylated enzyme than from the nonphosphorylated enzyme migrated with authentic L-glutamyl-gamma-propylhydroxamate in four chromatographic systems and on electrophoresis on paper at three different pH's. The acyl phosphate "intermediate" in the phosphorylated form of the adenosine-triphosphatase therefore appears to be an L-glutamyl-gamma-phosphate residue.

摘要

从脑微粒体钠钾激活的三磷酸腺苷酶制剂的磷酸化和非磷酸化形式经胃蛋白酶消化得到的肽段，在pH 5.4下用高比活性的N-（正丙基-2,3-（3）H）羟胺处理，然后通过柱色谱分离，再用链霉蛋白酶进一步消化。从磷酸化酶中分离出的一种化合物比从非磷酸化酶中分离出的量更多，在四种色谱系统以及在三种不同pH值下的纸上电泳中，它与纯L-谷氨酰-γ-丙基羟肟酸迁移情况相同。因此，三磷酸腺苷酶磷酸化形式中的酰基磷酸“中间体”似乎是一个L-谷氨酰-γ-磷酸残基。

相似文献

1
Sodium-potassium adenosine triphosphatase: acyl phosphate "intermediate" shown to be L-glutamyl-gamma-phosphate.钠钾腺苷三磷酸酶：酰基磷酸“中间体”被证明是L-谷氨酰-γ-磷酸。
Science. 1967 Jul 28;157(3787):434-6. doi: 10.1126/science.157.3787.434.
2
On the molecular characterization of the sodium-potassium transport adenosine triphosphatase.钠离子-钾离子转运三磷酸腺苷酶的分子特征。
J Gen Physiol. 1969 Jul 1;54(1):327-42. doi: 10.1085/jgp.54.1.327.
3
Evidence that a phosphorylated intermediate in a brain transport adenosine triphosphatase is an acyl phosphate.有证据表明，脑转运三磷酸腺苷酶中的磷酸化中间体是酰基磷酸。
Proc Natl Acad Sci U S A. 1965 Jul;54(1):177-84. doi: 10.1073/pnas.54.1.177.
4
Isolation and characterization of a phosphorylated intermediate in the (Na+ + K+) system-dependent ATPase.（Na⁺ + K⁺）系统依赖性ATP酶中磷酸化中间体的分离与鉴定。
Biochim Biophys Acta. 1966 Apr 12;118(1):106-15. doi: 10.1016/s0926-6593(66)80149-2.
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Studies on the characterization of the sodium-potassium transport adenosinetriphosphatase. II. Characterization of the acyl phosphate intermediate as an L-glutamyl-gamma-phosphate residue.钠钾转运三磷酸腺苷酶的特性研究。II. 酰基磷酸中间体作为L-谷氨酰-γ-磷酸残基的特性
Arch Biochem Biophys. 1968 Jul;126(1):331-42. doi: 10.1016/0003-9861(68)90589-4.
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Separation at neutral pH value of 32P-labelled proteins in a membrane preparation from ox brain. Partial characterization of component of the sodium-plus-potassium ion-activited adenosine triphosphatase.在中性pH值下对牛脑细胞膜制剂中32P标记蛋白质的分离。钠钾离子激活的三磷酸腺苷酶成分的部分特性分析。
Biochem J. 1974 Feb;137(2):253-62. doi: 10.1042/bj1370253.
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Lead-adenosine triphosphate complexes in adenosine triphosphatase histochemistry.三磷酸腺苷酶组织化学中的铅-三磷酸腺苷复合物
J Histochem Cytochem. 1969 Feb;17(2):85-94. doi: 10.1177/17.2.85.
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Purification of the sodium- and potassium-dependent adenosine triphosphatase from canine renal medulla.犬肾髓质中钠钾依赖型三磷酸腺苷酶的纯化
J Biol Chem. 1971 Jul 10;246(13):4157-65.
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The effect of chemical agents on the turnover of the bound phosphate associated with the sodium-and-potassium ion-stimulated adenosine triphosphatase in ox brain microsomes.化学试剂对牛脑微粒体中与钠钾离子刺激的三磷酸腺苷酶相关的结合磷酸盐周转的影响。
Biochem J. 1970 Nov;120(1):1-13. doi: 10.1042/bj1200001.
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Kinetic studies on a brain microsomal adenosine triphosphatase. II. Potassium-dependent phosphatase activity.脑微粒体三磷酸腺苷酶的动力学研究。II. 钾依赖性磷酸酶活性。
Biochemistry. 1969 Aug;8(8):3348-55. doi: 10.1021/bi00836a032.

引用本文的文献

1
Adenosinetriphosphate partition in cardiac muscle with respect to transmembrane electrical activity.
Pflugers Arch. 1971;322(2):95-108. doi: 10.1007/BF00592292.
2
Turnover of protein-bound phosphorylserine in membrane preparations from ox brain catalysed by intrinsic kinase and phosphatase activity.牛脑细胞膜制剂中结合蛋白的磷酸丝氨酸在内在激酶和磷酸酶活性催化下的周转。
Biochem J. 1971 Sep;124(2):393-406. doi: 10.1042/bj1240393.
3
DNP-induced dissipation of ATP in anoxic ventricular muscle.二硝基酚诱导的缺氧心室肌中三磷酸腺苷的耗散。
J Physiol. 1973 Mar;229(3):583-99. doi: 10.1113/jphysiol.1973.sp010155.
4
The effect of chemical agents on the turnover of the bound phosphate associated with the sodium-and-potassium ion-stimulated adenosine triphosphatase in ox brain microsomes.化学试剂对牛脑微粒体中与钠钾离子刺激的三磷酸腺苷酶相关的结合磷酸盐周转的影响。
Biochem J. 1970 Nov;120(1):1-13. doi: 10.1042/bj1200001.