牛脑细胞膜制剂中结合蛋白的磷酸丝氨酸在内在激酶和磷酸酶活性催化下的周转。
Turnover of protein-bound phosphorylserine in membrane preparations from ox brain catalysed by intrinsic kinase and phosphatase activity.
作者信息
Weller M, Rodnight R
出版信息
Biochem J. 1971 Sep;124(2):393-406. doi: 10.1042/bj1240393.
Abstract
- The turnover of protein-bound phosphorylserine in preparations of membrane fragments from ox brain cortex was studied. 2. Turnover was considered to arise from the action of intrinsic protein kinases and phosphatases on a membrane protein or proteins. 3. Properties of the kinase system were studied by measuring the rate of incorporation of (32)P from gamma-labelled ATP into protein-bound phosphorylserine isolated from partial acid hydrolysates of membrane proteins. 4. Properties of the phosphatase system were studied by observing the rate of loss of (32)P from membrane preparations pre-labelled with [(32)P]ATP. 5. Net phosphorylation and dephosphorylation of membrane protein was observed during incubation of membrane preparations with and without ATP. 6. The rate of turnover was about 4nmol of P/h per mg of protein at 20 degrees C; dephosphorylation was considered to be the rate-limiting step.
摘要
- 研究了牛脑皮质膜碎片制剂中蛋白质结合磷酸丝氨酸的周转率。2. 周转率被认为是由内在蛋白激酶和磷酸酶对一种或多种膜蛋白的作用引起的。3. 通过测量从膜蛋白部分酸水解产物中分离出的蛋白质结合磷酸丝氨酸中γ标记ATP的（32）P掺入率，研究了激酶系统的特性。4. 通过观察用[（32）P]ATP预标记的膜制剂中（32）P的损失率，研究了磷酸酶系统的特性。5. 在有和没有ATP的情况下孵育膜制剂时，观察到膜蛋白的净磷酸化和去磷酸化。6. 在20摄氏度时，周转率约为每毫克蛋白质每小时4nmol的P；去磷酸化被认为是限速步骤。
相似文献
1
Turnover of protein-bound phosphorylserine in membrane preparations from ox brain catalysed by intrinsic kinase and phosphatase activity.牛脑细胞膜制剂中结合蛋白的磷酸丝氨酸在内在激酶和磷酸酶活性催化下的周转。
Biochem J. 1971 Sep;124(2):393-406. doi: 10.1042/bj1240393.
2
The role of bound potassium ions in the hydrolysis of low concentrations of adenosine triphosphate by preparations of membrane fragments from ox brain cerebral cortex.结合钾离子在牛脑大脑皮质膜碎片制剂水解低浓度三磷酸腺苷中的作用。
Biochem J. 1970 Nov;120(1):15-24. doi: 10.1042/bj1200015.
3
Protein kinase activity in membrane preparations from ox brain. Stimulation of intrinsic activity by adenosine 3':5'-cyclic monophosphate.牛脑细胞膜制剂中的蛋白激酶活性。3':5'-环磷酸腺苷对内在活性的刺激作用。
Biochem J. 1973 Mar;132(3):483-92. doi: 10.1042/bj1320483.
4
The effect of chemical agents on the turnover of the bound phosphate associated with the sodium-and-potassium ion-stimulated adenosine triphosphatase in ox brain microsomes.化学试剂对牛脑微粒体中与钠钾离子刺激的三磷酸腺苷酶相关的结合磷酸盐周转的影响。
Biochem J. 1970 Nov;120(1):1-13. doi: 10.1042/bj1200001.
5
Enzyme transfer of phosphate from adenosine triphosphate to protein-bound serine residues in cerebral microsomes.磷酸从三磷酸腺苷转移至脑微粒体中与蛋白质结合的丝氨酸残基的酶促反应。
Biochem J. 1966 Nov;101(2):495-501. doi: 10.1042/bj1010495.
6
Protein-bound phosphorylserine in acid hydrolysates of brain tissue. The determination of ( 32 P)phosphorylserine by ion-exchange chromatography and electrophoresis.脑组织酸水解产物中的蛋白质结合磷酸丝氨酸。通过离子交换色谱法和电泳法测定（32P）磷酸丝氨酸。
Biochem J. 1971 Feb;121(4):597-600. doi: 10.1042/bj1210597.
7
Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity.脑微粒体的磷酸盐结合与三磷酸腺苷酶活性的关系。
Biochem J. 1966 Nov;101(2):502-15. doi: 10.1042/bj1010502.
8
Cyclic AMP-dependent reversible phosphorylation of renal medullary plasma membrane protein.
Endocrinology. 1972 Sep;91(3):757-63. doi: 10.1210/endo-91-3-757.
9
The influence of alkali metals on the incorporation of labelled phosphate into ATP in red cell ghosts.碱金属对标记磷酸盐掺入红细胞影中ATP的影响。
Biochim Biophys Acta. 1970 Jun 2;203(3):590-2. doi: 10.1016/0005-2736(70)90199-9.
10
A theoretical treatment of the turnover of protein-bound phosphate in the presence of both protein kinase and phosphatase activities.
Biochim Biophys Acta. 1974 May 24;343(3):565-83. doi: 10.1016/0304-4165(74)90275-x.
引用本文的文献
1
Cerebral ribosomal protein phosphorylation in experimental hyperphenylalaninaemia.实验性高苯丙氨酸血症中的脑核糖体蛋白磷酸化
Biochem J. 1980 Aug 15;190(2):405-19. doi: 10.1042/bj1900405.
2
GTP, as well as ATP, can act as a substrate for the intrinsic protein kinase activity of synaptic plasma membranes.三磷酸鸟苷（GTP）以及三磷酸腺苷（ATP）都可以作为突触质膜固有蛋白激酶活性的底物。
Mol Cell Biochem. 1981 Oct 30;40(2):75-85. doi: 10.1007/BF00224750.
3
Stimulation and inhibition by magnesium ions of intrinsic protein phosphorylating systems in synaptosomal membrane fragments from rat brain.镁离子对大鼠脑突触体膜碎片中内在蛋白磷酸化系统的刺激与抑制作用。
Neurochem Res. 1983 Nov;8(11):1441-9. doi: 10.1007/BF00964999.
4
Pre-incubation of subcellular fractions from rat cerebral cortex inactivates protein phosphorylation.
Biochem J. 1981 Oct 1;199(1):269-72. doi: 10.1042/bj1990269.
5
Characterization of protein phosphorylation in acetylcholine receptor-enriched membrane preparations from Torpedo fuscomaculata.斑纹电鳐富含乙酰胆碱受体的膜制剂中蛋白质磷酸化的特征分析。
Mol Cell Biochem. 1982 Feb 19;42(3):161-6. doi: 10.1007/BF00238510.
6
The soluble, cyclic AMP-stimulated protein kinase catalyses the phosphorylation of different membrane proteins from those which are phosphorylated by the membrane bound enzyme.可溶性的、受环磷酸腺苷刺激的蛋白激酶催化的膜蛋白磷酸化与膜结合酶催化的不同。
Mol Cell Biochem. 1981 Oct 30;40(2):95-104. doi: 10.1007/BF00224752.
7
The effect of putative transmitters and other agents on phosphoprotein turnover in respiring slices of guinea-pig cerebral cortex.假定递质和其他因子对豚鼠大脑皮层呼吸切片中磷蛋白周转的影响。
Biochem J. 1972 Feb;126(3):14P-15P. doi: 10.1042/bj1260014pb.
8
The state of phosphorylation in vivo of membrane-bound phosphoproteins in rat brain.大鼠脑中膜结合磷蛋白的体内磷酸化状态。
Biochem J. 1973 Jun;133(2):387-9. doi: 10.1042/bj1330387.
9
Protein kinase activity stimulated by adenosine 3' :5'-cyclic monophosphate in synaptic-membrane fragments from ox brain. Inhibition of intrinsic activity by free and membrane-bound calcium ions.3',5'-环磷酸腺苷刺激牛脑突触膜碎片中的蛋白激酶活性。游离和膜结合钙离子对内在活性的抑制作用。
Biochem J. 1974 Sep;142(3):605-9. doi: 10.1042/bj1420605.
10
Separation at neutral pH value of 32P-labelled proteins in a membrane preparation from ox brain. Partial characterization of component of the sodium-plus-potassium ion-activited adenosine triphosphatase.在中性pH值下对牛脑细胞膜制剂中32P标记蛋白质的分离。钠钾离子激活的三磷酸腺苷酶成分的部分特性分析。
Biochem J. 1974 Feb;137(2):253-62. doi: 10.1042/bj1370253.
本文引用的文献
1
The localization of cerebral phosphoprotein phosphatase.脑磷酸蛋白磷酸酶的定位
Biochem J. 1962 Jun;83(3):614-22. doi: 10.1042/bj0830614.
2
A phosphoprotein phosphatase from ox brain.来自牛脑的一种磷蛋白磷酸酶。
Biochem J. 1961 Nov;81(2):339-47. doi: 10.1042/bj0810339.
3
Molecular properties and transformations of glycogen phosphorylase in animal tissues.动物组织中糖原磷酸化酶的分子特性与转变
Adv Enzymol Relat Subj Biochem. 1962;24:263-90. doi: 10.1002/9780470124888.ch5.
4
Reversible phosphate transfer between yolk phosphoprotein and adenosine triphosphate.卵黄磷蛋白与三磷酸腺苷之间的可逆磷酸转移。
J Biol Chem. 1960 Apr;235:1043-50.
5
THE SUBCELLULAR LOCALIZATION OF CEREBRAL PHOSPHOPROTEINS SENSITIVE TO ELECTRICAL STIMULATION.对电刺激敏感的脑磷蛋白的亚细胞定位
Biochem J. 1965 Jun;95(3):889-96. doi: 10.1042/bj0950889.
6
THE BIOCHEMISTRY OF SODIUM TRANSPORT.钠转运的生物化学
Biol Rev Camb Philos Soc. 1964 May;39:160-93. doi: 10.1111/j.1469-185x.1964.tb00953.x.
7
Phosphoprotein metabolism and ion transport in nervous tissue: a suggested connexion.神经组织中的磷蛋白代谢与离子转运：一种推测的联系。
Nature. 1962 Feb 3;193:451-4. doi: 10.1038/193451a0.
8
Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.生物材料中的3',5'-磷酸腺苷。I. 环3',5'-核苷酸磷酸二酯酶的纯化及性质，以及用该酶鉴定人尿中的3',5'-磷酸腺苷
J Biol Chem. 1962 Apr;237:1244-50.
9
Nuclear phosphoproteins. II. Metabolism of exogenous phosphoprotein by intact nuclei.核磷蛋白。II. 完整细胞核对外源磷蛋白的代谢
Biochim Biophys Acta. 1969 Feb 4;175(1):136-41.
10
Nuclear phosphoproteins. I. Isolation and characterization of a phosphoprotein fraction from calf thymus nuclei.核磷蛋白。I. 从小牛胸腺核中分离和鉴定一种磷蛋白组分。
Biochim Biophys Acta. 1969 Feb 4;175(1):123-35.
Zotero 插件