Girard A, Ruckerbauer G M, Gray D P, Bannister G L, Boulanger P
Can J Comp Med Vet Sci. 1967 Jul;31(7):171-4.
Three different strains of bluetongue virus were adapted to grow in primary bovine foetal kidney cell cultures. The cytopathic effects observed from the three strains were similar, and were characterized by shrinkage of cells and increased granularity. The specificity of the changes was confirmed by the fluorescent antibody technique. No significant immunological cross-reaction was detected by serum-virus neutralization tests from the strains studied.
三种不同毒株的蓝舌病病毒被驯化在原代牛胎儿肾细胞培养物中生长。从这三种毒株观察到的细胞病变效应相似，其特征为细胞萎缩和颗粒度增加。通过荧光抗体技术证实了这些变化的特异性。在所研究的毒株中，血清-病毒中和试验未检测到明显的免疫交叉反应。
