劳斯氏白血病病毒蛋白质的化学特性分析

Chemical characterization of Rauscher leukaemia virus proteins.

作者信息

Brouwer J, Pluijms W J, Warnaar S O

出版信息

J Gen Virol. 1979 Feb;42(2):415-21. doi: 10.1099/0022-1317-42-2-415.

Abstract

The proteins of Rauscher murine leukaemia virus (R-MuLV) were characterized by amino acid analyses and by determination of their mol. wt. by gel filtration on cross-linked Sepharose 6B in 6M-guanidine hydrochloride (GuHCl). Molecular weights of 56,000, 29,000, 15,000, 10,500 and 7,600 were found for gp70, p30, p15, p12 and p10 respectively. The amino acid compositions of these proteins and of p12E have been determined. The amino acid compositions of the p10 polypeptides of Rauscher-MuLV and Moloney-MuLV are very similar as are those of the p30 polypeptides, whereas the amino acid compositions of the p12 polypeptides differ considerably. P12E contains the highest percentage of hydrophobic amino acid residues. Among the gag-gene coded proteins, p15 contains the highest percentage of hydrophobic amino acid residues while p12 and p10 contain the lowest.

摘要

劳氏鼠白血病病毒（R-MuLV）的蛋白质通过氨基酸分析以及在6M盐酸胍（GuHCl）中利用交联琼脂糖凝胶6B进行凝胶过滤测定其分子量来进行表征。分别发现gp70、p30、p15、p12和p10的分子量为56,000、29,000、15,000、10,500和7,600。已测定了这些蛋白质以及p12E的氨基酸组成。劳氏鼠白血病病毒和莫洛尼鼠白血病病毒的p10多肽的氨基酸组成非常相似，p30多肽的氨基酸组成也是如此，而p12多肽的氨基酸组成则有很大差异。P12E含有最高比例的疏水氨基酸残基。在gag基因编码的蛋白质中，p15含有最高比例的疏水氨基酸残基，而p12和p10含有最低比例。