Parks W P, Noon M C, Gilden R, Scolnick E M
J Virol. 1975 Jun;15(6):1385-95. doi: 10.1128/JVI.15.6.1385-1395.1975.
Major virion low-molecular-weight polypeptides were isolated from the Moloney strain of murine leukemia virus (type C) by agarose chromatography in 6M guanidine hydrochloride and were shown to have molecular weights of 15,000 (p15), 12,000 (p12), and 10,000 (p10) by their elution volumes and by their relative mobilities in sodium dodecyl sulfate-polyacrylamide gels. Each polypeptide could be iodinated and employed in double antibody radioimmunoassay procedures. All three polypeptides demonstrated a high degree of type-specificity in serologic immunoprecipitation analysis and in corresponding competition immunoassays. The p15 was immunologically distinct from other viron polypeptides including p12 and p10; the p12 and p10 were highly related to each other but not to other virion polypeptides and were even more type-specific than the p15 in serologic tests. Competition immunoassays with p15 and p10 indicate that the Moloney strain of MuLV is only a distant relative of the Friend-Rauscher group. The combined use of the Kirsten and Moloney low-molecular-weight polypeptide immunoassays suggest that xenotropic viruses constitute yet another group(s) of murine leukemia virus with distinct type-specific antigens, further expanding an already heterogeneous group of mouse type C viruses.
通过在6M盐酸胍中进行琼脂糖层析，从莫洛尼鼠白血病病毒（C型）中分离出主要病毒粒子低分子量多肽，并根据其洗脱体积和在十二烷基硫酸钠-聚丙烯酰胺凝胶中的相对迁移率，确定其分子量分别为15,000（p15）、12,000（p12）和10,000（p10）。每种多肽都可以进行碘化，并用于双抗体放射免疫分析程序。在血清学免疫沉淀分析和相应的竞争免疫分析中，所有这三种多肽都表现出高度的型特异性。p15在免疫学上与包括p12和p10在内的其他病毒粒子多肽不同；p12和p10彼此高度相关，但与其他病毒粒子多肽无关，并且在血清学检测中比p15具有更高的型特异性。用p15和p10进行的竞争免疫分析表明，莫洛尼鼠白血病病毒株只是弗瑞德-劳舍尔病毒组的远亲。结合使用柯斯顿和莫洛尼低分子量多肽免疫分析表明，嗜异性病毒构成了另一组具有独特型特异性抗原的鼠白血病病毒，进一步扩大了已经异质性的小鼠C型病毒组。
