Honigberg I L, Stewart J T, Coldren J W
J Pharm Sci. 1979 Feb;68(2):253-5. doi: 10.1002/jps.2600680239.
A method for the high-pressure liquid chromatographic determination of chlorzoxazone and its hydroxy metabolite in human plasma samples is presented. The separation of the compounds is achieved on an octadecylsilane column with a mobile phase of absolute methanol-distilled water (40:60) at a flow rate of 2.0 ml/min (3100 psig). The chromatographic separation is achieved within 10 min. The overall analysis time is about 45 min, which includes extraction of the drug and metabolite from plasma followed by high-pressure liquid chromatographic separation and quantification. The accuracy of the procedure is in the 1-5% range.
本文介绍了一种高压液相色谱法，用于测定人血浆样品中氯唑沙宗及其羟基代谢物。在十八烷基硅烷柱上进行化合物分离，流动相为无水甲醇-蒸馏水（40:60），流速为2.0 ml/min（3100 psig）。10分钟内实现色谱分离。整个分析时间约为45分钟，包括从血浆中提取药物和代谢物，然后进行高压液相色谱分离和定量。该方法的准确度在1%-5%范围内。
