Shimek J L, Rao N G, Khalil S K
J Pharm Sci. 1982 Apr;71(4):436-9. doi: 10.1002/jps.2600710415.
An isocratic high-pressure liquid chromatographic method for the determination of naproxen and its desmethyl metabolite in human plasma is presented. A reversed-phase octadecylsilane column was utilized with a mobile phase consisting of 55% methanol and 45% 0.10 M acetate buffer, pH 5.0. A spectrofluorometric detector with an excitation wavelength of 253 nm and a band pass filter provided high sensitivity with no interference from normal plasma constituents. The reproducibility and precision of the method were shown by analysis of spiked samples containing 2.5-70 micrograms/ml of plasma.
本文介绍了一种用于测定人血浆中萘普生及其去甲基代谢物的等度高压液相色谱法。使用反相十八烷基硅烷柱，流动相由55%甲醇和45% 0.10 M醋酸盐缓冲液（pH 5.0）组成。配备激发波长为253 nm的荧光检测器和带通滤光片，具有高灵敏度，不受正常血浆成分干扰。通过分析含2.5 - 70微克/毫升血浆的加标样品，展示了该方法的重现性和精密度。
