Hrudka F
Andrologia. 1979 Sep-Oct;11(5):337-53. doi: 10.1111/j.1439-0272.1979.tb02217.x.
A revised cytochemical procedure for demonstrating sperm oxidoreductases is presented in an attempt to standardize its use in comparative and experimental studies. Its detailed description includes improved incubation techniques, preparation of media, preservation of cytochemical activity on smears and evaluation of cytochemical preparations. Special attention is paid to the competing activities which may affect the specificity of the results.
本文介绍了一种用于显示精子氧化还原酶的改良细胞化学方法,旨在使其在比较研究和实验研究中的应用标准化。其详细描述包括改进的孵育技术、培养基的制备、涂片上细胞化学活性的保存以及细胞化学制剂的评估。特别关注了可能影响结果特异性的竞争活性。