[肝脏微粒体中各种受体与氧阴离子自由基的相互作用]
[Interaction of various acceptors with oxygen anion-radicals in liver microsomes].
作者信息
Mishin V M, Pokrovskiĭ A G, Liakhovich V V
出版信息
Biokhimiia. 1976 May;41(5):763-7.
PMID:828858
Abstract
Interaction of acceptors with oxygen (O2) anion-radicals appearing in the microsomal chain of electron transfers, was studied. It was found that nitro blue tetrazolium (NBT) was reduced in liver microsomes both in the presence of O2 and through direct acceptance of electrons. The values of Km and Vmax for NBT and epinephrine interactions with microsomes were determined. The rate of O2 generation was calculated from the data obtained.
摘要
研究了受体与微粒体电子传递链中出现的氧（O₂）阴离子自由基的相互作用。发现硝基蓝四唑（NBT）在肝微粒体中，无论是在有O₂存在的情况下还是通过直接接受电子，都会被还原。测定了NBT和肾上腺素与微粒体相互作用的Km和Vmax值。根据所得数据计算了O₂的生成速率。
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