Tyler D D, Gonze J, Lamy F, Dumont J E
Biochem J. 1968 Jan;106(1):123-33. doi: 10.1042/bj1060123.
The influence of mitochondrial inhibitors, including oligomycin, antimycin and rotenone, on the iodide and oxygen uptake and the nucleotide content of incubated sheep thyroid slices was investigated. Each inhibitor strongly suppressed both iodide and oxygen uptake, and decreased the nucleoside triphosphate content of the slices. In most cases the addition of glucose or mitochondrial substrates restored iodide uptake in inhibitor-treated slices. Inhibitor concentrations sufficient to inhibit iodide uptake strongly had only slight effects on the thyroidal Na(+)+K(+)-activated adenosine triphosphatase. It is concluded that the inhibitors produce their effects by the inhibition in vivo of mitochondrial oxidative phosphorylation. ATP synthesis appears to be essential for iodide uptake to occur, and the high-energy intermediates (or energized state) of oxidative phosphorylation cannot be used to energize the uptake process. To a limited extent glycolytic ATP synthesis can support iodide uptake, which is therefore not exclusively dependent on aerobic metabolism. The mechanism of energy-linked iodide uptake is discussed.
研究了包括寡霉素、抗霉素和鱼藤酮在内的线粒体抑制剂对孵育的绵羊甲状腺切片的碘摄取、氧摄取及核苷酸含量的影响。每种抑制剂均强烈抑制碘摄取和氧摄取，并降低切片中核苷三磷酸的含量。在大多数情况下，添加葡萄糖或线粒体底物可恢复抑制剂处理切片中的碘摄取。足以强烈抑制碘摄取的抑制剂浓度对甲状腺钠钾激活的三磷酸腺苷酶仅有轻微影响。得出的结论是，抑制剂通过在体内抑制线粒体氧化磷酸化发挥作用。ATP合成似乎是碘摄取发生所必需的，氧化磷酸化的高能中间体（或活跃状态）不能用于为摄取过程供能。糖酵解ATP合成在有限程度上可支持碘摄取，因此碘摄取并非完全依赖有氧代谢。文中讨论了能量关联碘摄取的机制。
