质子偶联转运系统的定量分析。大肠杆菌的乳糖通透酶。
Quantitative analysis of proton-linked transport systems. The lactose permease of Escherichia coli.
作者信息
Booth I R, Mitchell W J, Hamilton W A
出版信息
Biochem J. 1979 Sep 15;182(3):687-96. doi: 10.1042/bj1820687.
Abstract
Evidence is presented that lactose uptake into whole cells of Escherichia coli occurs by symport with a single proton over the range of external pH 6.5--7.7. The proton/lactose stoicheiometry has been measured directly over this pH range by comparison of the initial rates of proton and lactose uptake into anaerobic resting cell suspensions of E. coli ML308. Further, the relationship between the protonmotive force and lactose accumulation has been studied in E. coli ML308-225 over the range of external pH 5.9--8.7. At no point was the accumulation of the beta-galactoside in thermodynamic equilibrium with the protonmotive force. It is concluded that the concentration of lactose within the cell is governed by kinetic factors rather than pH-dependent changes in the proton/substrate stoicheiometry. The relevance of these findings to the model of pH-dependent proton/substrate stoicheiometries derived from studies with E. coli membrane vesicles is discussed.
摘要
有证据表明，在外部pH值为6.5 - 7.7的范围内，大肠杆菌全细胞摄取乳糖是通过与单个质子协同运输实现的。通过比较大肠杆菌ML308厌氧静止细胞悬液中质子和乳糖的初始摄取速率，直接测定了该pH范围内的质子/乳糖化学计量比。此外，还研究了在外部pH值为5.9 - 8.7的范围内，大肠杆菌ML308 - 225中质子动力与乳糖积累之间的关系。在任何时候，β-半乳糖苷的积累都与质子动力不存在热力学平衡。得出的结论是，细胞内乳糖的浓度受动力学因素控制，而非质子/底物化学计量比中pH依赖性的变化。讨论了这些发现与从大肠杆菌膜囊泡研究中得出的pH依赖性质子/底物化学计量比模型的相关性。
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