饮食和激素条件对大鼠附睾脂肪组织中糖酵解酶活性的影响。

The effect of dietary and hormonal conditions on the activities of glycolytic enzymes in rat epididymal adipose tissue.

作者信息

Saggerson E D, Greenbaum A L

出版信息

Biochem J. 1969 Nov;115(3):405-17. doi: 10.1042/bj1150405.

DOI:10.1042/bj1150405

PMID:4242855

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185119/

Abstract

Measurements were made of the activities of nine glycolytic enzymes in epididymal adipose tissues obtained from rats that had undergone one of the following treatments: starvation; starvation followed by re-feeding with bread or high-fat diet; feeding with fat without preliminary starvation; alloxan-diabetes; alloxan-diabetes followed by insulin therapy. 2. In general, the activities of the glycolytic enzymes of adipose tissue, unlike those of liver, were not greatly affected by the above treatments. 3. The ;key' glycolytic enzymes, phosphofructokinase and pyruvate kinase, were generally no more adaptive in response to physiological factors than other glycolytic enzymes such as glucose phosphate isomerase, fructose diphosphate aldolase, triose phosphate isomerase, glycerol 3-phosphate dehydrogenase, phosphoglycerate kinase and lactate dehydrogenase. 4. Adiposetissue pyruvate kinase did not respond to feeding with fat in a manner similar to the liver enzyme. 5. Glyceraldehyde phosphate dehydrogenase had a behaviour pattern unlike the other eight glycolytic enzymes studied in that its activity was depressed by feeding with fat and was not restored to normal by re-feeding with a high-fat diet after starvation. These results are discussed in relation to the requirements of adipose tissue for glycerol phosphate in the esterification of fatty acids. 6. A statistical analysis of the results permitted the writing of linear equations describing the relationships between the activities of eight of the enzymes studied. 7. Evidence is presented for the existence of two constant-proportion groups amongst the enzymes studied, namely (i) glucose phosphate isomerase, phosphoglycerate kinase and lactate dehydrogenase, and (ii) triose phosphate isomerase, fructose diphosphate aldolase and pyruvate kinase. 8. Mechanisms for maintaining the observed relationships between the activities of the enzymes in the tissue are discussed.

摘要

对以下几种处理方式的大鼠附睾脂肪组织中的九种糖酵解酶活性进行了测定：饥饿；饥饿后再喂食面包或高脂饮食；未预先饥饿直接喂食脂肪；四氧嘧啶糖尿病；四氧嘧啶糖尿病后进行胰岛素治疗。2. 一般来说，与肝脏不同，上述处理方式对脂肪组织中糖酵解酶的活性影响不大。3. “关键”糖酵解酶，磷酸果糖激酶和丙酮酸激酶，在对生理因素的反应上，通常并不比其他糖酵解酶如葡萄糖磷酸异构酶、果糖二磷酸醛缩酶、磷酸丙糖异构酶、甘油3 - 磷酸脱氢酶、磷酸甘油酸激酶和乳酸脱氢酶更具适应性。4. 脂肪组织丙酮酸激酶对喂食脂肪的反应与肝脏酶不同。5. 磷酸甘油醛脱氢酶的行为模式与所研究的其他八种糖酵解酶不同，其活性因喂食脂肪而降低，饥饿后再喂食高脂饮食也不能恢复正常。结合脂肪酸酯化过程中脂肪组织对磷酸甘油的需求对这些结果进行了讨论。6. 对结果进行统计分析后得出了描述所研究的八种酶活性之间关系的线性方程。7. 有证据表明在所研究的酶中存在两个恒定比例组，即（i）葡萄糖磷酸异构酶、磷酸甘油酸激酶和乳酸脱氢酶，以及（ii）磷酸丙糖异构酶、果糖二磷酸醛缩酶和丙酮酸激酶。8. 讨论了维持组织中酶活性之间观察到的关系的机制。