圆二色性与DNA二级结构
Circular dichroism and DNA secondary structure.
作者信息
Baase W A, Johnson W C
出版信息
Nucleic Acids Res. 1979 Feb;6(2):797-814. doi: 10.1093/nar/6.2.797.
Abstract
The change in average rotation of the DNA helix has been determined for the transfer from 0.05 M NaCl to 3.0 M CsCl, 6.2 M LiCl and 5.4 M NH4Cl. This work, combined with data at lower salt from other laboratories, allows us to relate the intensity of the CD of DNA at 275 nm directly to the change in the number of base pairs per turn. The change in secondary structure for the transfer of DNA from 0.05 M NaCl (where it is presumably in the B-form) to high salt (where the characteristic CD has been interpreted as corresponding to C-form geometry) is found to be -0.22 (+/- 0.02) base pairs per turn. In the case of mononucleosomes, where the CD indicates the "C-form", the change in secondary structure (including temperature effects) would add -0.31 (+/- 0.03) turns about the histone core to the -1.25 turns estimated from work on SV40 chromatin. Accurate winding angles and molar extinction coefficients were determined for ethidium.
摘要
已测定了DNA螺旋平均旋转角度在从0.05 M NaCl转移至3.0 M CsCl、6.2 M LiCl和5.4 M NH4Cl时的变化。这项工作，结合其他实验室在低盐浓度下的数据，使我们能够将DNA在275 nm处的圆二色性强度直接与每圈碱基对数目的变化联系起来。发现DNA从0.05 M NaCl（推测为B型）转移至高盐（其特征性圆二色性被解释为对应于C型结构）时，二级结构的变化为每圈-0.22（±0.02）个碱基对。在单核小体的情况下，其圆二色性表明为“C型”，二级结构的变化（包括温度效应）会使围绕组蛋白核心的圈数在根据SV40染色质研究估计的-1.25圈基础上再增加-0.31（±0.03）圈。已确定了溴化乙锭的精确缠绕角度和摩尔消光系数。
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