体内溴化乙锭及体外与脱氧核糖核酸结合的溴化乙锭的荧光检测圆二色性

Fluorescence-detected circular dichroism of ethidium in vivo and bound to deoxyribonucleic acid in vitro.

作者信息

Lamos M L, Turner D H

出版信息

Biochemistry. 1985 Jun 4;24(12):2819-22. doi: 10.1021/bi00333a001.

Abstract

Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However, the bands at 325 and 385 nm disappear when ethidium binds to macromolecules without intercalating between base pairs. The results demonstrate that FDCD spectra can be measured in cell suspensions and indicate that ethidium binds to nucleic acids in E. coli cells by intercalation.

摘要

报道了在大肠杆菌细胞中以及体外与大肠杆菌DNA结合的溴化乙锭的荧光检测圆二色性（FDCD）光谱。在325和385nm处观察到FDCD谱带。随着溴化乙锭与DNA比例的变化，这些谱带的幅度会发生改变。体内和体外测量的光谱相似。然而，当溴化乙锭与大分子结合而不插入碱基对之间时，325和385nm处的谱带消失。结果表明，可以在细胞悬液中测量FDCD光谱，并且表明溴化乙锭通过插入作用与大肠杆菌细胞中的核酸结合。