牛血清、初乳和哺乳犊牛中的大肠杆菌凝集素。
Escherichia coli agglutinins in cow serum, colostrum and the nursing calf.
作者信息
Jacks T M, Glantz P J
出版信息
Can J Comp Med. 1970 Jul;34(3):213-7.
Immunochemical properties of Escherichia coli O antibodies present in bovine serum and colostrum were investigated. Dam and calf serum samples plus colostral whey samples were fractionated by gel filtration, and the 7S and 19S fractions isolated. Antibody activity against the O antigens of four recognized E. coli bovine pathogens was determined by the indirect hemagglutination test on the whole serum and colostral whey samples and the 7S and 19S fractions thereof. Mercaptoethanol reduction was used to chemically study the immunochemistry of the E. coli O antibodies. The E. coli O antibodies in dam serum were entirely 19S macroglobulins and appeared to be IgM immunoglobulins. The antibodies in colostrum and calf serum were both 7S and 19S globulins. Reasons for believing these 7S antibodies may be IgG, and the 19S antibodies IgA, immunoglobulins are presented.
对牛血清和初乳中存在的大肠杆菌O抗体的免疫化学特性进行了研究。对母畜和犊牛血清样本以及初乳乳清样本进行凝胶过滤分级分离，分离出7S和19S组分。通过对全血清、初乳乳清样本及其7S和19S组分进行间接血凝试验，测定了针对四种公认的大肠杆菌牛病原体O抗原的抗体活性。使用巯基乙醇还原法对大肠杆菌O抗体的免疫化学进行化学研究。母畜血清中的大肠杆菌O抗体完全是19S巨球蛋白，似乎是IgM免疫球蛋白。初乳和犊牛血清中的抗体既有7S球蛋白也有19S球蛋白。文中给出了认为这些7S抗体可能是IgG、19S抗体可能是IgA免疫球蛋白的理由。
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