从细胞培养物中生长的衣原体（鸟疫）病原体提取的补体结合抗原和血凝抗原。
Complement-fixation and hemagglutination antigens from a Chlamydial (ornithosis) agent grown in cell cultures.
作者信息
Grimes J E, Grumbles L C, Moore R W
出版信息
Can J Comp Med. 1970 Jul;34(3):256-60.
Methods are described for the preparation of complement-fixation (CF) and hemagglutination (HA) antigens from the Texas turkey ornithosis agent grown in McCoy cell culture monolayers. The particulate antigens prepared for this study were satisfactory for testing mammalian sera by direct CF tests and avian sera by indirect CF or modified direct CF tests. Comparison of titers were made on human, bovine and ovine sera using direct CF tests employing antigen prepared for this study, 6 BC yolk sac antigen, and a commercially available antigen. The HA antigen agglutinated mouse erythrocytes, but it was not of value in hemagglutination inhibition tests because of "nonspecific" inhibitors in both mammalian and avian sera.
本文描述了从在 McCoy 细胞培养单层中生长的德克萨斯火鸡鸟疫病原体制备补体结合（CF）和血凝（HA）抗原的方法。本研究制备的颗粒性抗原通过直接 CF 试验检测哺乳动物血清以及通过间接 CF 或改良直接 CF 试验检测禽类血清的效果令人满意。使用为本研究制备的抗原、6BC 卵黄囊抗原和一种市售抗原，通过直接 CF 试验对人、牛和羊血清的滴度进行了比较。HA 抗原能凝集小鼠红细胞，但由于哺乳动物和禽类血清中存在“非特异性”抑制剂，它在血凝抑制试验中没有价值。
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