衣原体抗体间接血凝试验
Indirect hemagglutination test for chlamydial antibodies.
作者信息
Lewis V J, Thacker W L, Engelman H M
出版信息
Appl Microbiol. 1972 Jul;24(1):22-5. doi: 10.1128/am.24.1.22-25.1972.
Abstract
An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the complement fixation test. Lymphogranuloma venereum antibodies also were detected by using antigen extracted from C. psittaci.
摘要
本文描述了一种用于鹦鹉热诊断血清中衣原体抗体的间接血凝试验（IHA）；该试验使用经在Vero细胞单层中生长的鹦鹉热衣原体脱氧胆酸盐提取物致敏的鞣酸处理的绵羊红细胞。将IHA试验适配至微量滴定系统会降低灵敏度；尽管如此，微量滴定-IHA试验仍比补体结合试验更灵敏。通过使用从鹦鹉热衣原体中提取的抗原也可检测到性病性淋巴肉芽肿抗体。
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The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera.蛋白质在经单宁酸处理的红细胞上的吸附以及随后抗蛋白质血清引起的血细胞凝集。
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