一种淋巴细胞冷冻保存的简单方法。冷冻保存的细胞对特定免疫效应功能的保留。

A simple method for the cryopreservation of lymphocytes. Retention of specific immune effector functions by frozen-stored cells.

作者信息

Grant C K

出版信息

Clin Exp Immunol. 1976 Jan;23(1):166-74.

PMID:4251

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1538356/

Abstract

A simple, quick and inexpensive method for cryostorage of lymphocytes is discribed. When injected into appropriate normal recipients, frozen-stored rat and sheep lymphocytes caused GVH and NLT reactions respectively. Sheep lymphocytes remained viable after several months storage and functioned satisfactorily as 51Cr-labelled target cells in assays for cytotoxic antibodies. Lymphocytes, including immunoblasts, drained from sheep efferent lymphatics during immune responses to injected murine P815 cells or allogeneic lymphocytes survived freezing; when thawed the cells retained specific immune cytotoxic effector functions including the ability to secrete complement-dependent and leucocyte-dependent antibodies

摘要

本文描述了一种简单、快速且廉价的淋巴细胞冷冻保存方法。当将冷冻保存的大鼠和绵羊淋巴细胞注射到合适的正常受体中时，分别引发了移植物抗宿主反应和非淋巴细胞性甲状腺炎反应。绵羊淋巴细胞在储存数月后仍保持活力，并在细胞毒性抗体检测中作为51Cr标记的靶细胞发挥了令人满意的作用。在对注射的小鼠P815细胞或同种异体淋巴细胞的免疫反应过程中，从绵羊输出淋巴管中引流出来的淋巴细胞，包括免疫母细胞，在冷冻后仍能存活；解冻后的细胞保留了特异性免疫细胞毒性效应功能，包括分泌补体依赖性和白细胞依赖性抗体的能力

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A simple method for the cryopreservation of lymphocytes. Retention of specific immune effector functions by frozen-stored cells.一种淋巴细胞冷冻保存的简单方法。冷冻保存的细胞对特定免疫效应功能的保留。

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8

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10

Graft-vs-host reactions (GVHR) across minor murine histocompatibility barriers. II. Development of natural suppressor cell activity.跨越小鼠次要组织相容性屏障的移植物抗宿主反应（GVHR）。II. 天然抑制细胞活性的发展。

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本文引用的文献

1

The recirculation of lymphocytes from blood to lymph in the rat.大鼠体内淋巴细胞从血液到淋巴的再循环。

J Physiol. 1959 Apr 23;146(1):54-69. doi: 10.1113/jphysiol.1959.sp006177.

2

Characteristics of lymphocyte transfer reactions produced in sheep.绵羊体内产生的淋巴细胞转移反应的特征

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3

The preservation of white cells.白细胞的保存

Cryobiology. 1968 Jul-Aug;5(1):70-5. doi: 10.1016/s0011-2240(68)80146-4.

4

Cryoprotective agents.冷冻保护剂

Cryobiology. 1971 Apr;8(2):173-83. doi: 10.1016/0011-2240(71)90024-1.

5

Comparative study of the effectiveness of dimethyl sulfoxide and polyvinylpyrrolidone in the preservation of human leukemic blood cells at--80 degrees C.二甲基亚砜与聚乙烯吡咯烷酮在-80℃保存人白血病血细胞有效性的比较研究

Cryobiology. 1970 Mar-Apr;6(5):409-15. doi: 10.1016/s0011-2240(70)80098-0.

6

The role of cooling rates in low-temperature preservation.冷却速率在低温保存中的作用。

Cryobiology. 1971 Oct;8(5):447-52. doi: 10.1016/0011-2240(71)90035-6.

7

Some variations in lymphocyte freezing methods which do not affect cell viability.一些不影响细胞活力的淋巴细胞冷冻方法的变化。

Tissue Antigens. 1972;2(1):20-6. doi: 10.1111/j.1399-0039.1972.tb00113.x.

8

Optimal recovery of lymphocytes and tissue culture cells following rapid cooling.快速冷却后淋巴细胞和组织培养细胞的最佳恢复情况。

Nature. 1974 May 31;249(456):452-3. doi: 10.1038/249452a0.

9

A bank of frozen peripheral blood lymphocytes for in vitro immunological studies on East African cancer patients.一批用于东非癌症患者体外免疫学研究的冷冻外周血淋巴细胞。

Int J Cancer. 1974 Apr 15;13(4):489-93. doi: 10.1002/ijc.2910130407.

10

Use of different cooling rates during freezing to separate populations of human peripheral blood lymphocytes.在冷冻过程中使用不同的冷却速率来分离人外周血淋巴细胞群体。

Cryobiology. 1972 Dec;9(6):516-25. doi: 10.1016/0011-2240(72)90173-3.

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