Issekutz T B, Chin G W, Hay J B
Clin Exp Immunol. 1981 Sep;45(3):604-14.
Afferent lymphatics draining granulomas and efferent lymphatics from normal lymph nodes were cannulated in sheep. Cells collected from these lymphatics were radiolabelled in vitro with 111In (afferent lymph cells) and 51Cr (efferent lymphocytes) and both labelled cells were returned to the animal simultaneously by i.v. injection. The reappearance of these labelled cells in lymph, and the amount of 111In and 51Cr in normal or antigenically stimulated lymph nodes, cutaneous inflammatory sites (FCA-granulomas, NLT- and BCG-induced lesions) and blood was determined 24 hr later. As previously reported, labelled afferent cells preferentially migrated from the blood back through the granuloma into afferent lymph, and efferent lymphocytes back into efferent lymph. Forty per cent as many 111In- as 51Cr-labelled cells ;appeared in efferent lymph. This was caused by the greater migration of 51Cr-labelled cells appeared in efferent lymph. This was caused by the greater migration of 51Cr- than 111In-labelled cells out of the blood into the node. Neither cell type was selectively retained in the node, and 28% of the labelled cells that entered the node migrated on into efferent lymph in 24 hr. Similarly, there was no selective retention of either cell type in the granuloma, and equal amounts of 111In the 51Cr appeared in afferent lymph. The ratio 111In/51Cr in the blood suggested that in the lymph node the two labelled cell populations were extracted equally, while in the granuloma selectively at the level of the vascular endothelium resulted in the preferential extraction of 111In-labelled (afferent lymph) cells.
在绵羊身上，将引流肉芽肿的输入淋巴管和来自正常淋巴结的输出淋巴管插管。从这些淋巴管收集的细胞在体外分别用铟 - 111（输入淋巴细胞）和铬 - 51（输出淋巴细胞）进行放射性标记，然后通过静脉注射将两种标记细胞同时回输到动物体内。24小时后，测定这些标记细胞在淋巴液中的再次出现情况，以及在正常或抗原刺激的淋巴结、皮肤炎症部位（弗氏完全佐剂肉芽肿、非特异性淋巴细胞转移和卡介苗诱导的病变）和血液中的铟 - 111和铬 - 51含量。如先前报道，标记的输入细胞优先从血液中回流，经肉芽肿进入输入淋巴管，而输出淋巴细胞则回流到输出淋巴管。出现在输出淋巴管中的铟 - 111标记细胞数量仅为铬 - 51标记细胞数量的40%。这是由于铬 - 51标记细胞比铟 - 111标记细胞从血液中迁移到淋巴结中的数量更多所致。两种细胞类型在淋巴结中均未被选择性滞留，进入淋巴结的标记细胞中有28%在24小时内迁移至输出淋巴管。同样，两种细胞类型在肉芽肿中均未被选择性滞留，且等量的铟 - 111和铬 - 51出现在输入淋巴管中。血液中铟 - 111与铬 - 51的比例表明，在淋巴结中，两种标记细胞群体被同等程度地摄取，而在肉芽肿中，血管内皮水平的选择性摄取导致铟 - 111标记（输入淋巴）细胞被优先摄取。
