钠和钾对兔心室肌切片摄取间羟胺的影响。

The ionic dependence of [(3)H]-metaraminol transport by rabbit ventricular slices was studied.2. Transport was Na(+) dependent. Choline, Li(+), K(+), Rb(+) or Cs(+) could not be substituted for Na(+).3. Transport was K(+) dependent. Rb(+) and Cs(+), but not Li, could be substituted for K(+), their relative potencies being K(+) = or >Rb(+)>Cs(+)>>Li(+). Higher concentrations of K(+), Rb(+), Cs(+) and Li inhibited [(3)H]-metaraminol transport.4. The inhibitory effects on transport of either a marked reduction in the concentration of Na(+) or of omission of K(+) were rapidly reversible on exposure of slices to Krebs solution.5. The inhibitory effect of ouabain on [(3)H]-metaraminol transport was markedly time dependent, being significantly increased by preincubation with ouabain. The inhibitory action of ouabain was not affected, however, by the Na(+) concentration present during preincubation.6. An inwardly directed Na(+) gradient did not increase [(3)H]-metaraminol transport in slices in which Na(+) pumping had been prevented by 10(-5)M ouabain, 4 degrees C, omission of K(+) or by metabolic inhibition.7. These findings provide additional evidence that the Na(+) and K(+) activated adenosine triphosphatase may participate in the transport of metaraminol and related amines. In the absence of Na(+) pumping, induced Na(+) gradients were unable to produce transport of amine as would be predicted by the co-transport model proposed by Bogdanski & Brodie (1969).

研究了家兔心室切片对[³H] - 间羟胺转运的离子依赖性。
转运依赖于Na⁺。胆碱、Li⁺、K⁺、Rb⁺或Cs⁺不能替代Na⁺。
转运依赖于K⁺。Rb⁺和Cs⁺可替代K⁺，而Li⁺不能，它们的相对效力为K⁺ = 或 >Rb⁺>Cs⁺>>Li⁺。较高浓度的K⁺、Rb⁺、Cs⁺和Li⁺会抑制[³H] - 间羟胺的转运。
当切片暴露于Krebs溶液时，Na⁺浓度显著降低或K⁺缺失对转运的抑制作用迅速逆转。
哇巴因对[³H] - 间羟胺转运的抑制作用明显具有时间依赖性，预孵育哇巴因会使其显著增强。然而，预孵育期间存在的Na⁺浓度并不影响哇巴因的抑制作用。
在10⁻⁵M哇巴因、4℃、K⁺缺失或代谢抑制阻止了Na⁺泵浦的切片中，内向的Na⁺梯度并未增加[³H] - 间羟胺的转运。
这些发现提供了额外的证据，表明Na⁺和K⁺激活的三磷酸腺苷酶可能参与间羟胺及相关胺类的转运。在没有Na⁺泵浦的情况下，诱导的Na⁺梯度无法产生胺类的转运，这与Bogdanski和Brodie（1969年）提出的协同转运模型的预测一致。

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices.