Ellman L, Green I, Judge F, Frank M M
J Exp Med. 1971 Jul 1;134(1):162-75. doi: 10.1084/jem.134.1.162.
Guinea pigs with a genetically determined total deficiency of the fourth component of complement have been studied for various in vivo immunological functions. Passive cutaneous anaphylaxsis, contact and delayed hypersensitivity, and the cellular exudative response to a foreign body were normal. These animals also have normal direct and reverse passive Arthus reactions which suggest that they possess a mechanism to bypass C4 and directly activate late-acting complement components. This would appear to be an unequivocal demonstration of an alternate pathway in the complement sequence. Immune clearance of guinea pig erythrocytes sensitized with rabbit antibody was impaired in the deficient animals. Antibody production in C4-deficient animals was impaired for two of the three antigens studied.
对补体第四成分存在基因决定的完全缺陷的豚鼠进行了多种体内免疫功能研究。被动皮肤过敏反应、接触性和迟发型超敏反应以及对异物的细胞渗出反应均正常。这些动物的直接和反向被动阿瑟斯反应也正常,这表明它们拥有一种绕过C4并直接激活晚期补体成分的机制。这似乎明确证明了补体序列中的替代途径。在缺陷动物中,用兔抗体致敏的豚鼠红细胞的免疫清除受损。在研究的三种抗原中,有两种抗原在C4缺陷动物中的抗体产生受损。