Hall R E, Blaese R M, Davis A E, Decker J M, Tack B F, Colten H R, Muchmore A V
J Exp Med. 1982 Sep 1;156(3):834-43. doi: 10.1084/jem.156.3.834.
Synergistic cytotoxicity is a term used to describe a cytotoxic system in which xenogeneic erythrocyte target cells are lysed in the presence of nonimmune human mononuclear effector cells and antibody-depleted normal human serum. Neither the mononuclear cells nor the serum alone are cytolytic to the target erythrocytes. Previous studies have shown that the serum activity is not immunoglobulin and is heat-labile, suggesting a similarity to serum complement. In this report, sera deficient in various complement components as well as highly purified single complement components were tested with whole mononuclear cell populations and purified monocytes and lymphocytes to further characterize this cytotoxicity system. Whole mononuclear cell populations failed to mediate target cell lysis in sera deficient in C5 or factor B. However, C3-deficient serum, even in the presence of anti-C3 antibody, supported synergistic cytotoxicity normally. Purified lymphocytes were also normally cytotoxic in C3-deficient serum but failed to lyse targets in sera deficient in C5, C7, C8, or depleted of factor B. Purified monocytes failed to lyse the target cells only in factor B-depleted serum and could lyse the target cells in serum-free medium when purified factor B alone was added. Monocyte-mediated cytotoxicity induced by factor B was inhibited 73-100% by adding lymphocytes back to the purified monocytes. Thus, both lymphocytes and monocytes can serve as effector cells in this form of cytotoxicity but require cooperative interaction with different sets of complement components. In addition, lymphocytes can modulate the monocyte-mediated form of target cell lysis associated with factor B.
协同细胞毒性是一个用于描述细胞毒性系统的术语，在该系统中，异种红细胞靶细胞在非免疫性人单核效应细胞和抗体缺失的正常人血清存在的情况下被裂解。单核细胞和血清单独都不会对靶红细胞产生细胞溶解作用。先前的研究表明，血清活性不是免疫球蛋白，且对热不稳定，这表明它与血清补体相似。在本报告中，用缺乏各种补体成分的血清以及高度纯化的单一补体成分，对全单核细胞群体以及纯化的单核细胞和淋巴细胞进行了测试，以进一步表征这个细胞毒性系统。在缺乏C5或B因子的血清中，全单核细胞群体无法介导靶细胞裂解。然而，缺乏C3的血清，即使存在抗C3抗体，通常也能支持协同细胞毒性。纯化的淋巴细胞在缺乏C3的血清中也具有正常的细胞毒性，但在缺乏C5、C7、C8或缺乏B因子的血清中无法裂解靶细胞。纯化的单核细胞仅在缺乏B因子的血清中无法裂解靶细胞，当单独添加纯化的B因子时，在无血清培养基中能够裂解靶细胞。通过将淋巴细胞重新添加到纯化的单核细胞中，B因子诱导的单核细胞介导的细胞毒性被抑制了73% - 100%。因此，淋巴细胞和单核细胞都可以作为这种细胞毒性形式的效应细胞，但需要与不同组的补体成分进行协同相互作用。此外，淋巴细胞可以调节与B因子相关的单核细胞介导的靶细胞裂解形式。
