Welch A B
Can J Comp Med. 1971 Jul;35(3):195-202.
Studies have been conducted on a virus which has recently been implicated as an etiological agent in widely disseminated cases of calf diarrhea. The virus was isolated from feces and intestinal mucosa from experimentally infected calves and from cell cultures which had been inoculated with material from infected calves. The virus was purified by differential centrifugation, nuclease treatment, extraction with Genetron 113 and cesium chloride (CsC1) gradient. Purified and non-purified samples were studied by electron microscopy. Both types of preparations contained unenveloped particles approximately 64 nm in diameter with a hexagonal core region 36 nm in diameter. Subunits of the capsids appeared to be arranged in accordance with cubic synmetry. Essential lipids were not associated with the virus. The buoyant density of purified virus was 1.359. Viral nucleic acid was determined by chemical methods to be ribonucleic acid (RNA). The morphology and some chemical characteristics appear to be similar to those of the reovirus group. However, the exact classification of this virus and its relationship to other groups remains to be established.
针对一种病毒开展了多项研究，该病毒最近被认为是犊牛腹泻广泛传播病例中的病原体。从实验感染犊牛的粪便和肠黏膜以及接种了感染犊牛材料的细胞培养物中分离出了这种病毒。通过差速离心、核酸酶处理、用Genetron 113和氯化铯（CsC1）梯度提取对病毒进行了纯化。通过电子显微镜对纯化和未纯化的样本进行了研究。两种制剂均含有直径约64nm的无包膜颗粒，其核心区域为直径36nm的六边形。衣壳亚基似乎按照立方对称排列。病毒不与必需脂质相关。纯化病毒的浮力密度为1.359。通过化学方法确定病毒核酸为核糖核酸（RNA）。其形态和一些化学特征似乎与呼肠孤病毒组相似。然而，这种病毒的确切分类及其与其他组的关系仍有待确定。
