牛心磷酸果糖激酶的同位素交换反应。
The isotope-exchange reactions of ox heart phosphofructokinase.
作者信息
Hulme E C, Tipton K F
出版信息
Biochem J. 1971 Apr;122(2):181-7. doi: 10.1042/bj1220181.
Abstract
- Ox heart phosphofructokinase catalyses isotope-exchange reactions at pH6.7 between ADP and ATP, and between fructose 6-phosphate and fructose 1,6-diphosphate, the latter reaction being absolutely dependent on the presence of the magnesium complex of ADP. 2. The reaction kinetics are hyperbolic with respect to substrate concentration for both exchange reactions (within the experimental error). 3. The influence of pH, AMP and citrate suggests that the fructose 6-phosphate-fructose 1,6-diphosphate exchange is subject to effector control, and is abolished by dissociation of the enzyme. 4. These results are discussed in relation to the reaction mechanism of the enzyme.
摘要
- 牛心磷酸果糖激酶在pH6.7时催化ADP与ATP之间以及6-磷酸果糖与1,6-二磷酸果糖之间的同位素交换反应，后一反应绝对依赖于ADP的镁配合物的存在。2. 对于两个交换反应，反应动力学相对于底物浓度呈双曲线（在实验误差范围内）。3. pH、AMP和柠檬酸的影响表明，6-磷酸果糖-1,6-二磷酸果糖交换受效应物控制，并且在酶解离时被消除。4. 结合该酶的反应机制对这些结果进行了讨论。
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本文引用的文献
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