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与三磷酸腺苷依赖性脱氧核糖核酸酶相关的三磷酸腺苷酶（recB-recC酶 - 大肠杆菌 - 三磷酸腺苷至磷酸二酯水解比率 - 依赖于DNA的三磷酸腺苷酶活性）

Adenosine triphosphatase associated with adenosine triphosphate-dependent deoxyribonuclease (recB-recC enzyme-E. coli-ATP to phosphodiester hydrolysis ratio-DNA-dependent ATPase activity).

作者信息

Nobrega F G, Rola F H, Pasetto-Nobrega M, Oishi M

出版信息

Proc Natl Acad Sci U S A. 1972 Jan;69(1):15-9. doi: 10.1073/pnas.69.1.15.

DOI:10.1073/pnas.69.1.15
PMID:4257817
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427534/
Abstract

An ATPase activity that is completely dependent on DNA is associated with the ATP-dependent DNase (recB-recC enzyme) purified from Escherichia coli. There is a strong correlation between the ATPase and the DNase activities under various assay conditions. With E. coli DNA as substrate, 8-9 molecules of ATP are hydrolyzed to ADP and inorganic phosphate for every phosphodiester bond hydrolyzed by the DNase. The possible functional relationship of the ATPase and DNase activities is discussed.

摘要

一种完全依赖于DNA的ATP酶活性与从大肠杆菌中纯化得到的依赖ATP的DNA酶（recB-recC酶）相关。在各种测定条件下，ATP酶活性和DNA酶活性之间存在很强的相关性。以大肠杆菌DNA为底物时，DNA酶每水解一个磷酸二酯键，就有8 - 9个ATP分子水解为ADP和无机磷酸。文中讨论了ATP酶活性和DNA酶活性可能的功能关系。

相似文献

1
Adenosine triphosphatase associated with adenosine triphosphate-dependent deoxyribonuclease (recB-recC enzyme-E. coli-ATP to phosphodiester hydrolysis ratio-DNA-dependent ATPase activity).与三磷酸腺苷依赖性脱氧核糖核酸酶相关的三磷酸腺苷酶（recB-recC酶 - 大肠杆菌 - 三磷酸腺苷至磷酸二酯水解比率 - 依赖于DNA的三磷酸腺苷酶活性）
Proc Natl Acad Sci U S A. 1972 Jan;69(1):15-9. doi: 10.1073/pnas.69.1.15.
2
Reconstitution of RecBC DNase activity from purified Escherichia coli RecB and RecC proteins.从纯化的大肠杆菌RecB和RecC蛋白中重建RecBC核酸酶活性。
J Biol Chem. 1985 Jan 25;260(2):1224-9.
3
On the role of ATP in phosphodiester bond hydrolysis catalyzed by the recBC deoxyribonuclease of Escherichia coli.关于ATP在大肠杆菌recBC脱氧核糖核酸酶催化的磷酸二酯键水解中的作用。
J Biol Chem. 1977 Jan 25;252(2):499-503.
4
Substrate specificity and adenosine triphosphatase activity of the ATP-dependent deoxyribonuclease of Bacillus subtilis.枯草芽孢杆菌ATP依赖型脱氧核糖核酸酶的底物特异性和三磷酸腺苷酶活性
Eur J Biochem. 1981 Mar;114(3):493-9. doi: 10.1111/j.1432-1033.1981.tb05172.x.
5
An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination.一种来自大肠杆菌的依赖ATP的脱氧核糖核酸酶，可能在基因重组中发挥作用。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292-9. doi: 10.1073/pnas.64.4.1292.
6
An adenosine triphosphate dependent deoxyribonuclease with adenosine triphosphatase, activity from Bacillus cereus.一种来自蜡样芽孢杆菌的具有三磷酸腺苷酶活性的依赖三磷酸腺苷的脱氧核糖核酸酶。
Acta Biochim Biophys Acad Sci Hung. 1979;14(1-2):53-66.
7
An alternative approach to the study of new enzymatic reactions involving DNA (DNA-dependent ATPases-purification-properties-E. coli).一种研究涉及DNA的新酶促反应的替代方法（依赖DNA的ATP酶——纯化——特性——大肠杆菌）。
Proc Natl Acad Sci U S A. 1972 Feb;69(2):515-9. doi: 10.1073/pnas.69.2.515.
8
A reduced activity of a deoxyribonuclease requiring ATP in Escherichia coli infected by bacteriophage T4.在被噬菌体T4感染的大肠杆菌中，一种需要ATP的脱氧核糖核酸酶的活性降低。
Biochim Biophys Acta. 1971 Nov 19;247(4):535-41. doi: 10.1016/0005-2787(71)90690-3.
9
The recBC deoxyribonuclease of Escherichia coli: isolation and characterization of the subunit proteins and reconstitution of the enzyme.大肠杆菌的recBC脱氧核糖核酸酶：亚基蛋白的分离与特性鉴定及酶的重组
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4816-20. doi: 10.1073/pnas.71.12.4816.
10
Separation of ATP-dependent DNAse to ATPase and DNAse.将依赖ATP的DNA酶分离为ATP酶和DNA酶。
Acta Biol Acad Sci Hung. 1981;32(1):45-54.

引用本文的文献

1
RecBCD enzyme and the repair of double-stranded DNA breaks.RecBCD酶与双链DNA断裂的修复
Microbiol Mol Biol Rev. 2008 Dec;72(4):642-71, Table of Contents. doi: 10.1128/MMBR.00020-08.
2
DNA-replication of spi - mutants of bacteriophage lambda in recombination-deficient bacteria.噬菌体λ的spi-突变体在重组缺陷型细菌中的DNA复制
Mol Gen Genet. 1972;117(3):229-38. doi: 10.1007/BF00271650.
3
Differential thermolability of exonuclease and endonuclease activities of the recBC nuclease isolated from thermosensitive recB and recC mutants.从温度敏感型recB和recC突变体中分离出的recBC核酸酶的核酸外切酶和核酸内切酶活性的差异热稳定性。
J Bacteriol. 1974 Dec;120(3):1219-22. doi: 10.1128/jb.120.3.1219-1222.1974.
4
Fate of conjugally transferred DNA in minicells of Escherichia coli K-12.大肠杆菌K-12小细胞中接合转移DNA的命运
Mol Gen Genet. 1974;128(1):23-42. doi: 10.1007/BF00267292.
5
The recBC deoxyribonuclease of Escherichia coli: isolation and characterization of the subunit proteins and reconstitution of the enzyme.大肠杆菌的recBC脱氧核糖核酸酶：亚基蛋白的分离与特性鉴定及酶的重组
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4816-20. doi: 10.1073/pnas.71.12.4816.
6
DNA degradation in minicells of Escherichia coli K-12. II. Effect of recA1 and recB21 mutations on DNA degradation in minicells and detection of exonuclease V activity.大肠杆菌K-12微小细胞中的DNA降解。II. recA1和recB21突变对微小细胞中DNA降解的影响及外切核酸酶V活性的检测
Mol Gen Genet. 1975 Jun 19;138(3):179-92. doi: 10.1007/BF00269345.
7
DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli.在大肠杆菌提取物中，DNA合成涉及DNA聚合酶I的一种复合形式。
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2099-103. doi: 10.1073/pnas.72.6.2099.
8
Postinfection control by bacteriophage T4 of Escherichia coli recBC nuclease activity.噬菌体T4对大肠杆菌recBC核酸酶活性的感染后控制
J Virol. 1976 Apr;18(1):20-5. doi: 10.1128/JVI.18.1.20-25.1976.
9
Partial purification and properties of a bacteriophage T7 inhibitor of the host exonuclease V activity.宿主核酸外切酶V活性的噬菌体T7抑制剂的部分纯化及性质
J Virol. 1975 Nov;16(5):1200-7. doi: 10.1128/JVI.16.5.1200-1207.1975.
10
Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec+ and rec minus spheroplasts measured with different forms of bacteriophage DNA.大肠杆菌原生质体的转染。V. 用不同形式的噬菌体DNA测定rec⁺和rec⁻原生质体中recBC核酸酶的活性。
J Virol. 1975 Apr;15(4):861-71. doi: 10.1128/JVI.15.4.861-871.1975.

本文引用的文献

1
The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA.甲醛与核苷酸及T2噬菌体DNA的反应。
J Mol Biol. 1961 Feb;3:47-60. doi: 10.1016/s0022-2836(61)80007-7.
2
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
3
The DNA of bacteriophage PM 2. Ultracentrifugal evidence for a circular structure.噬菌体PM 2的DNA。关于环状结构的超速离心证据。
Virology. 1969 Mar;37(3):495-8. doi: 10.1016/0042-6822(69)90238-4.
4
DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes.DNA修复与基因重组：对在这些过程中存在缺陷的大肠杆菌突变体的研究。
Radiat Res. 1966:Suppl 6:156+.
5
An endonuclease activity from Escherichia coli absent from certain rec- strains.来自大肠杆菌的一种核酸内切酶活性，某些rec-菌株中不存在该活性。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):434-41. doi: 10.1073/pnas.67.1.434.
6
An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination.一种来自大肠杆菌的依赖ATP的脱氧核糖核酸酶，可能在基因重组中发挥作用。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292-9. doi: 10.1073/pnas.64.4.1292.
7
Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.大肠杆菌中重组能力的生化与遗传学研究。I. 与recB+和recC+基因相关的酶活性。
Proc Natl Acad Sci U S A. 1970 Apr;65(4):955-61. doi: 10.1073/pnas.65.4.955.
8
A deoxyribonuclease which requires nucleoside triphosphate from Micrococcus lysodeikticus. II. Studies on the role of nucleoside triphosphate.一种来自溶壁微球菌、需要三磷酸核苷的脱氧核糖核酸酶。II. 三磷酸核苷作用的研究
J Biol Chem. 1970 Feb 25;245(4):775-80.