大肠杆菌中重组能力的生化与遗传学研究。I. 与recB+和recC+基因相关的酶活性。
Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.
作者信息
Barbour S D, Clark A J
出版信息
Proc Natl Acad Sci U S A. 1970 Apr;65(4):955-61. doi: 10.1073/pnas.65.4.955.
Abstract
An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains. Mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nuclease activity. The over-all reaction in crude lysates digests native DNA to nucleoside monophosphates. Complementation between recB21 and recC22 in vivo leads to normal levels of ATP-dependent nuclease activity. No complementation in vitro has been detected. Mutations in a third recombination gene (recA) do not alter significantly the wild-type levels of this nuclease activity.
摘要
在recB(+)recC(+)菌株的裂解物中检测到一种依赖三磷酸腺苷的脱氧核糖核酸酶活性。recB或recC中的突变会导致这种活性丧失，这表明这两个基因决定了核酸酶活性。粗裂解物中的总体反应将天然DNA消化为单磷酸核苷。recB21和recC22在体内的互补作用导致ATP依赖的核酸酶活性达到正常水平。在体外未检测到互补作用。第三个重组基因（recA）中的突变不会显著改变这种核酸酶活性的野生型水平。
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