Gartner L P, Seibel W, Hiatt J L, Provenza D V
Acta Anat (Basel). 1979;103(1):16-33. doi: 10.1159/000144994.
The first mandibular molars of the Swiss albino mice, 1 through 4 days of age, were fixed in glutaraldehyde or Karnovsky's fixative. The tissues were postfixed in OSO4, dehydrated and embedded in Epon. The prepolarizing, polarizing and secretory odontoblasts were described. The prepolarizing cells, located in the vicinity of the cervical loop, were mesenchymal-like in morphology. The cells of the polarizing stage possessed organelles indicative of protein synthesis. The nucleus was located proximally. Aperiodic fibers were evident in the wide basement membrane. The secretory odontoblasts were long, slender, polarized cells closely adjoining one another. Each odontoblast possessed six morphologically discernible regions: (1) an infranuclear region, limited in size and containing few cellular organelles; (2) a nuclear region, housing the oval nucleus and a few associated lamellae of rough endoplasmic reticulum as well as a limited number of mitochondria; (3) a supranuclear rough endoplasmic reticulum region, possessing an abundance of these organelles as well as some mitochondria and secretory vesicles; (4) a Golgi region, occupying the middle third of the cell, housing the elements of an extensive Golgi apparatus which was surrounded by peripherally located profiles of rough endoplasmic reticulum; additionally, this region contained smooth endoplasmic reticulum, mitochondria, numerous secretory granules and vesicles and occasional intracellular collagen fibers; (5) an apical rough endoplasmic reticulum region, containing a rough endoplasmic reticulum component that was less extensive than its supranuclear counterpart; in addition, this region was the one richest in mitochondria and contained a plethora of secretory vesicles and granules; (6) the odontoblastic process, a region mostly void of organelles, containing various secretory products, some of which appeared to be in the process of being released extracellularly into the surrounding dentin matrix.
将1至4日龄的瑞士白化小鼠的第一下颌磨牙用戊二醛或卡诺夫斯基固定液固定。组织用四氧化锇后固定，脱水并包埋在环氧树脂中。描述了前极化、极化和分泌期成牙本质细胞。位于颈环附近的前极化细胞在形态上类似间充质细胞。极化期的细胞具有指示蛋白质合成的细胞器。细胞核位于近端。在宽阔的基底膜中有周期性纤维。分泌期成牙本质细胞是长而细的极化细胞，彼此紧密相邻。每个成牙本质细胞有六个形态上可辨别的区域：(1)核下区域，体积有限，含有少量细胞器；(2)核区域，容纳椭圆形细胞核和一些相关的粗面内质网板层以及数量有限的线粒体；(3)核上粗面内质网区域，含有大量这些细胞器以及一些线粒体和分泌小泡；(4)高尔基体区域，占据细胞的中间三分之一，容纳广泛的高尔基体成分，其周围被粗面内质网的周边轮廓包围；此外，该区域含有滑面内质网、线粒体、大量分泌颗粒和小泡以及偶尔的细胞内胶原纤维；(5)顶端粗面内质网区域，含有比核上对应区域不太广泛的粗面内质网成分；此外，该区域是线粒体最丰富的区域，含有大量分泌小泡和颗粒；(6)成牙本质细胞突，一个大部分没有细胞器的区域，含有各种分泌产物，其中一些似乎正在细胞外释放到周围的牙本质基质中。
