Pick E, Krejci J, Turk J L
Immunology. 1972 Jan;22(1):25-37.
Guinea-pigs immunized with sheep red cells (SRC) in Freund's complete adjuvant (FCA) developed delayed type hypersensitivity to a SRC urea extract 7 days after sensitization and showed combined Arthus and delayed reactivity from the 14th day on. Animals immunized by an intracardiac injection of SRC in saline and skin tested 4–5 days later with SRC urea extract, responded with an erythematous and indurated lesion reaching its peak 24 hr after testing and resembling a typical delayed hypersensitivity reaction. Lymphocytes from lymph nodes, peritoneal exudate and peripheral blood of guinea-pigs immunized with SRC in FCA released skin reactive factor (SRF) and macrophage migration inhibitory factor (MIF) when cultured for 24 hr with SRC. No SRF was formed in the absence of divalent cations in the cultured medium. No SRF or MIF was produced by lymphocytes of guinea-pigs immunized by the intracardiac route and cultured under identical conditions. SRF obtained after exposure of sensitized lymphocytes to SRC was eluted from Sephadex G-200 in the region of serum albumin. Lytic anti-SRC antibody of the IgM class was liberated into the supernatant of spleen cultures, after intracardiac immunization and antibody of the IgG class was produced by lymph node cultures after immunization with SRC in FCA, in both cases in the absence of antigenic stimulation . It is considered improbable that the latter antibody is involved in the release of soluble mediators.
用弗氏完全佐剂（FCA）中的绵羊红细胞（SRC）免疫的豚鼠在致敏7天后对SRC尿素提取物产生迟发型超敏反应，并从第14天开始表现出阿瑟斯反应和迟发反应的联合反应。通过心内注射生理盐水溶解的SRC免疫动物，并在4 - 5天后用SRC尿素提取物进行皮肤试验，动物出现红斑和硬结病变，在试验后24小时达到峰值，类似于典型的迟发型超敏反应。用FCA中的SRC免疫的豚鼠的淋巴结、腹腔渗出液和外周血中的淋巴细胞在与SRC一起培养24小时时释放皮肤反应因子（SRF）和巨噬细胞移动抑制因子（MIF）。在培养基中不存在二价阳离子的情况下不形成SRF。通过心内途径免疫并在相同条件下培养的豚鼠淋巴细胞不产生SRF或MIF。致敏淋巴细胞暴露于SRC后获得的SRF在血清白蛋白区域从Sephadex G - 200上洗脱下来。心内免疫后，IgM类的溶细胞抗SRC抗体释放到脾细胞培养上清液中，在用FCA中的SRC免疫后，淋巴结培养产生IgG类抗体，在这两种情况下均无抗原刺激。认为后一种抗体不太可能参与可溶性介质的释放。
