McGill D J, Dawes E A
Biochem J. 1971 Dec;125(4):1059-68. doi: 10.1042/bj1251059.
Isotopic and enzymic evidence indicates that Zymomonas anaerobia ferments glucose via the Entner-Doudoroff pathway. The molar growth yields with glucose (5.89) and fructose (5.0) are lower than those for the related organism Zymomonas mobilis and the observed linear growth suggests that energetically uncoupled growth occurs. A survey of enzymes of carbohydrate metabolism revealed the presence of weak phosphofructokinase and fructose 1,6-diphosphate aldolase activities but phosphoketolase, transketolase and transaldolase were not detected. Fermentation balances for glucose and fructose are reported; acetaldehyde accumulated in both fermentations, to a greater extent with fructose which also yielded glycerol and dihydroxyacetone as minor products.
同位素和酶学证据表明,厌氧发酵单胞菌通过恩特纳-杜德洛夫途径发酵葡萄糖。葡萄糖(5.89)和果糖(5.0)的摩尔生长产率低于相关生物运动发酵单胞菌,观察到的线性生长表明发生了能量解偶联生长。对碳水化合物代谢酶的调查显示存在弱磷酸果糖激酶和果糖1,6-二磷酸醛缩酶活性,但未检测到磷酸酮醇酶、转酮醇酶和转醛醇酶。报道了葡萄糖和果糖的发酵平衡;两种发酵中都积累了乙醛,果糖发酵中乙醛积累程度更高,果糖还产生了少量甘油和二羟基丙酮。